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Articles 1 - 30 of 52
Full-Text Articles in Bioresource and Agricultural Engineering
Spatial Distributions Of Heating, Cooling, And Industrial Degree-Days In Turkey, Ilhami Yildiz, B. Sosaoglu
Spatial Distributions Of Heating, Cooling, And Industrial Degree-Days In Turkey, Ilhami Yildiz, B. Sosaoglu
BioResource and Agricultural Engineering
The degree-day method is commonly used to estimate energy consumption for heating and cooling in residential, commercial and industrial buildings, as well as in greenhouses, livestock facilities, storage facilities and warehouses. This article presents monthly and yearly averages and spatial distributions of heating, cooling, and industrial degree-days at the base temperatures of 18 °C and 20 °C, 18 °C and 24 °C, and 7 °C and 13 °C, respectively; as well as the corresponding number of days in Turkey. The findings presented here will facilitate the estimation of heating and cooling energy consumption for any residential, commercial and industrial buildings …
Biomass And Biofuels In Maine: Estimating Supplies For Expanding The Forest Products Industry, Jonathan Rubin, Kate Dickerson, Jacob Kavkewitz
Biomass And Biofuels In Maine: Estimating Supplies For Expanding The Forest Products Industry, Jonathan Rubin, Kate Dickerson, Jacob Kavkewitz
Energy & the Environment
This paper estimates the renewable energy potential of Maine’s forest resources, and how much energy these resources could potentially provide the state. Using the most recent state-specific data available, and a methodology similar to the Billion Tons Report, we find that ethanol production from Maine’s forest residues could potentially provide 18% of Maine’s transportation (gasoline) fuels with a fermentation wood to ethanol process. Making Fischer-Tropsch diesel (F-T diesel) using forest residues can replace 39% of Maine’s petro-diesel consumption. Actual levels of biofuels that can be produced will depend on conversion factors and forestry residue removals that are subject to uncertainty.
Manure Testing: What To Request?, Charles A. Shapiro, Charles S. Wortmann
Manure Testing: What To Request?, Charles A. Shapiro, Charles S. Wortmann
Department of Biological Systems Engineering: Papers and Publications
Manure testing is necessary to make optimum use of manure while protecting water resources. This publication is a guide to providing information on a Manure Sample Submission Form for reliable interpretation of results.
The tests most frequently needed to optimize nutrient management are total and ammonium nitrogen (NH4-N), phosphorus (P2O5), potassium (K2O), pH, soluble salts, sodium, and dry matter content.
Nitrogen. Manure contains both organic and inorganic forms of nitrogen. Ammonium-N is the primary inorganic form in manure and is readily available to crops. Nitrate-N concentration is usually too small to …
Evaluation Of Irrigation Strategies With The Dssat Cropping System Model, Derek M. Heeren, Hal D. Warner, Todd P. Trooien
Evaluation Of Irrigation Strategies With The Dssat Cropping System Model, Derek M. Heeren, Hal D. Warner, Todd P. Trooien
Department of Biological Systems Engineering: Papers and Publications
Water is becoming an increasingly valuable commodity with shortages and water rationing more commonplace. Since irrigation is the largest consumptive use of water in South Dakota, accounting for over 70% of the water withdrawals, irrigation water management is critical to make the best use of the water available. This project uses the CERES-Maize cropping system model (available in DSSAT v4) to study the impact of various irrigation management strategies on corn production. SDSU management software developed by Oswald (2006) is used to simulate a center pivot for specific locations and years. Weather data from several sites in the Great Plains …
A Site-Wide Perspective On Uranium Geochemistry At The Hanford Site, John M. Zachara, C. Brown, J. Christensen, J.A. Davis, E. Dresel, Chongxuan Liu, S. Kelly, James Mckinley, J. Serne, W. Um
A Site-Wide Perspective On Uranium Geochemistry At The Hanford Site, John M. Zachara, C. Brown, J. Christensen, J.A. Davis, E. Dresel, Chongxuan Liu, S. Kelly, James Mckinley, J. Serne, W. Um
US Department of Energy Publications
Uranium (U) is an important risk-driving contaminant at the Hanford Site. Over 200,000 kg have been released to the vadose zone over the course of site operations, and a number of vadose zone and groundwater plumes containing the uranyl cation [UO22+, U(VI)] have been identified. U is recognized to be of moderate-to-high mobility, conditions dependent. The site is currently making decisions on several of these plumes with long-lasting implications, and others are soon to come.
Uranium is one of nature’s most intriguing and chemically complex elements. The fate and transport of U(VI) has been studied over the …
Scenarios Of Greenhouse Gas Emissions And Atmospheric Concentrations, Leon Clarke, James Edmonds, Henry Jacoby, Hugh Pitcher, John Reilly, Richard Richels
Scenarios Of Greenhouse Gas Emissions And Atmospheric Concentrations, Leon Clarke, James Edmonds, Henry Jacoby, Hugh Pitcher, John Reilly, Richard Richels
US Department of Energy Publications
This and a companion report constitute one of twenty-one Synthesis and Assessment Products called for in the Strategic Plan for the U.S. Climate Change Science Program. These studies are structured to provide high-level, integrated research results on important science issues with a particular focus on questions raised by decision-makers on dimensions of climate change directly relevant to the U.S. One element of the CCSP’s strategic vision is to provide decision support tools for differentiating and evaluating response strategies. Scenario-based analysis is one such tool. The scenarios in this report explore the implications of alternative stabilization levels of anthropogenic greenhouse …
Global-Change Scenarios: Their Development And Use, Edward Parson, Virginia Burkett, Karen Fisher-Vanden, David Keith, Linda Mearns, Hugh Pitcher, Cynthia Rosenzweig, Mort Webster
Global-Change Scenarios: Their Development And Use, Edward Parson, Virginia Burkett, Karen Fisher-Vanden, David Keith, Linda Mearns, Hugh Pitcher, Cynthia Rosenzweig, Mort Webster
US Department of Energy Publications
A scenario is a description of potential future conditions produced to inform decision-making under uncertainty. Scenarios can help inform decisions that involve high stakes and poorly characterized uncertainty, which may thwart other, conventional forms of analysis or decision support. Originally developed to study military and security problems, scenarios are now widely used for strategic planning and assessment in businesses and other organizations, and increasingly to inform planning, analysis, and decision-making for environmental issues, including climate change.
Scenarios can serve many purposes. They can help inform specific decisions, or can provide inputs to assessments, models, or other decision-support activities when these …
Laying The Groundwork For Odor Control And Setback Estimation In Nebraska, Rick R. Stowell
Laying The Groundwork For Odor Control And Setback Estimation In Nebraska, Rick R. Stowell
Department of Biological Systems Engineering: Papers and Publications
The goal of this field research study was to validate use of the Odor Footprint Tool (OFT) with livestock building sources in rural communities. The primary objective was to evaluate the accuracy of predictions of annoyance potential (as projected by odor concentration output data from AERMOD® - the OFT's underlying dispersion model) when compared to measures of annoyance potential made in the field. Odor assessors were trained to monitor odors around a 4,800-head finishing site in eastern Nebraska. During the summer of 2005, mobile odor assessors monitored odor levels at downwind locations. During the spring and summer of 2006, …
Volumetric Irrigation Water Pricing Considerations, Charles M. Burt
Volumetric Irrigation Water Pricing Considerations, Charles M. Burt
BioResource and Agricultural Engineering
Volumetric water pricing is a popular topic within donor agencies for irrigation project modernization and sustainability. Implementation of an effective pricing program is quite complex and requires consideration of physical modernization, fee structure, enforcement procedures, and the level of water delivery service. Variations of volumetric water pricing and allocation are discussed.
Corn Stover Availability And Collection Efficiency Using Typical Hay Equipment, Robert M. Prewitt, Michael D. Montross, Scott A. Shearer, Timothy S. Stombaugh, Stephen F. Higgins, Samuel G. Mcneill, Shahab Sokhansanj
Corn Stover Availability And Collection Efficiency Using Typical Hay Equipment, Robert M. Prewitt, Michael D. Montross, Scott A. Shearer, Timothy S. Stombaugh, Stephen F. Higgins, Samuel G. Mcneill, Shahab Sokhansanj
Biosystems and Agricultural Engineering Faculty Publications
Corn stover has been identified as a potential feedstock for the production of fermentable sugars and thermochemical processes. The availability and efficiency of typical hay equipment for collecting corn stover has not been well quantified. Corn stover was collected for two years on a central Kentucky farm near Louisville. Six different harvesting treatments, using traditional hay equipment, were used to harvest corn stover. A rotary mower, rotary scythe (flail-type mower with windrow-forming shields), parallel bar rake, and a round baler were utilized. The average stover moisture content prior to grain harvest was above 40%, and field drying was required before …
Dairy Waste Utilization Management Tool, José R. Bicudo, Anshu Singh
Dairy Waste Utilization Management Tool, José R. Bicudo, Anshu Singh
Agricultural Engineering Extension Publications
Quick tests enabling rapid, on‑farm assessment of manure nutrient content can significantly enhance manure management practices. Rapid or quick tests are less accurate than standard laboratory analyses but can be useful as a manure utilization tool.
Characterization Of Dairy Milk House Waste Water In Kentucky, Anshu Singh, Czarena L. Crofcheck, Gail M. Brion
Characterization Of Dairy Milk House Waste Water In Kentucky, Anshu Singh, Czarena L. Crofcheck, Gail M. Brion
Biosystems and Agricultural Engineering Faculty Publications
This study focuses on characterization of milk house waste water from eight different farms in Kentucky. The farms were separated into three groups based on the number of cows: small (20-30), medium (30-60), and large (over 60 cows). Samples were collected once a month from four farms and twice a month from the remainder. Samples were analyzed for chemical, biochemical, and microbiological characteristics. Results indicated a large and significant variation in the chemical and microbiological characteristics between the farms. Farm size had a significant effect on the nutrient content of the waste water. Though samples exhibited seasonal variation, there was …
Flexible Irrigation Systems: Concept, Design And Application, John L. Merriam, Stuart W. Styles, Beau J. Freeman
Flexible Irrigation Systems: Concept, Design And Application, John L. Merriam, Stuart W. Styles, Beau J. Freeman
BioResource and Agricultural Engineering
This paper presents the need, value, and concept of flexible irrigation water supply systems that can deliver water with flexibility in frequency, rate, and duration under the control of the farmer at the point of application using a limited rate arranged-demand or other schedule. It introduces the needed terminology including “congestion” — how much reserve time and capacity is required to assure water delivery at the frequency and rate desired. An illustrative design procedure for the necessary pipeline and reservoir capacities is illustrated. The techniques discussed emphasize the conversion of the economical steady supply canal flows to flexible on-farm usage …
Intellectual Property Management In Health And Agricultural Innovation: A Handbook Of Best Practices, Vol. 2, Anatole Krattiger, Richard T. Mahoney, Lita Nelsen, Jennifer A. Thomson, Alan B. Bennett, Kanikaram Satyanarayana, Gregory D. Graff, Carlos Fernandez, Stanley Kowalski
Intellectual Property Management In Health And Agricultural Innovation: A Handbook Of Best Practices, Vol. 2, Anatole Krattiger, Richard T. Mahoney, Lita Nelsen, Jennifer A. Thomson, Alan B. Bennett, Kanikaram Satyanarayana, Gregory D. Graff, Carlos Fernandez, Stanley Kowalski
Law Faculty Scholarship
Prepared by and for policy-makers, leaders of public sector research establishments, technology transfer professionals, licensing executives, and scientists, this online resource offers up-to-date information and strategies for utilizing the power of both intellectual property and the public domain. Emphasis is placed on advancing innovation in health and agriculture, though many of the principles outlined here are broadly applicable across technology fields. Eschewing ideological debates and general proclamations, the authors always keep their eye on the practical side of IP management. The site is based on a comprehensive Handbook and Executive Guide that provide substantive discussions and analysis of the opportunities …
Controlled Covalent Functionalization Of Multiwalled Carbon Nanotubes Using [4 + 2] Cycloaddition Of Benzocyclobutenes, Georgios Sakellariou, Haining Ji, Jimmy W. Mays, Nikos Hadjichristidis, Durairaj Baskaran
Controlled Covalent Functionalization Of Multiwalled Carbon Nanotubes Using [4 + 2] Cycloaddition Of Benzocyclobutenes, Georgios Sakellariou, Haining Ji, Jimmy W. Mays, Nikos Hadjichristidis, Durairaj Baskaran
US Department of Energy Publications
Surface modification of carbon nanotubes (CNTs) through covalent functionalization is vital for the development of high-performance composite materials, chemosensors, nanoelectronics, photovoltaic devices, as well as for a range of biomedical applications. Several methods have been developed to functionalize CNTs. The introduction of acid groups by acid digestion disrupts the structural integrity of CNTs. Apart from shortening the tubes, oxidatively generated acid groups are inhomogenously located at the tips of broken CNTs and, hence, functionalization using acid groups as precursors does not give a statistical distribution of functional groups throughout the surface of the CNTs.
Biostimulation Of Iron Reduction And Subsequent Oxidation Of Sediment Containing Fe-Silicates And Fe-Oxides: Effect Of Redox Cycling On Fe(Iii) Bioreduction, John Komlos, Ravi K. Kukkadapu, John M. Zachara, Peter R. Jaffe
Biostimulation Of Iron Reduction And Subsequent Oxidation Of Sediment Containing Fe-Silicates And Fe-Oxides: Effect Of Redox Cycling On Fe(Iii) Bioreduction, John Komlos, Ravi K. Kukkadapu, John M. Zachara, Peter R. Jaffe
US Department of Energy Publications
Sediment containing a mixture of iron (Fe)-phases, including Fe-oxides (mostly Al-goethite) and Fe-silicates (illites and vermiculite) was bioreduced in a long-term flow through column experiment followed by re-oxidation with dissolved oxygen. The objective of this study was (a) to determine the nature of the re-oxidized Fe(III), and (b) to determine how redox cycling of Fe would affect subsequent Fe(III)-bioavailability. In addition, the effect of Mn on Fe(III) reduction was explored.57Fe-Mössbauer spectroscopy measurements showed that biostimulation resulted in partial reduction (20%) of silicate Fe(III) to silicate Fe(II) while the reduction of goethite was negligible. Furthermore, the reduction of Fe …
Influence Of Calcium On Microbial Reduction Of Solid Phase Uranium(Vi), Chongxuan Liu, Byong-Hun Jeon, John M. Zachara, Zheming Wang
Influence Of Calcium On Microbial Reduction Of Solid Phase Uranium(Vi), Chongxuan Liu, Byong-Hun Jeon, John M. Zachara, Zheming Wang
US Department of Energy Publications
The effect of calcium on the dissolution and microbial reduction of a representative solid phase uranyl [U(VI)], sodium boltwoodite (NaUO2SiO3OH •1.5H2O), was investigated to evaluate the rate-limiting step of microbial reduction of the solid phase U(VI). Microbial reduction experiments were performed in a culture of a dissimilatory metal-reducing bacterium (DMRB), Shewanella oneidensis strain MR-1, in a bicarbonate medium with lactate as electron donor at pH 6.8 buffered with PIPES. Calcium increased the rate of Na-boltwoodite dissolution and U(VI) bioavailability by increasing its solubility through the formation of a ternary aqueous calcium-uranyl-carbonate species. The ternary …
Hanford Site Vadose Zone Studies: An Overview, G. W. Gee, M. Oostrom, M. D. Freshley, M. L. Rockhold, John M. Zachara
Hanford Site Vadose Zone Studies: An Overview, G. W. Gee, M. Oostrom, M. D. Freshley, M. L. Rockhold, John M. Zachara
US Department of Energy Publications
Large quantities of radioactive and chemical wastes resulting from Pu production for nuclear weapons are located in the vadose zone at the USDOE’s Hanford Site, north of Richland, WA. The vadose zone here is characterized by often highly stratified glacial-fluvial sediments that give rise to complex subsurface-flow paths that contribute to uncertainty of contaminant fate and transport. Research efforts have focused on answering questions of contaminant transport from the viewpoint of geologic, biologic, geochemical, and hydrologic controls. This special section highlights key research topics concerning vadose zone problems at the Hanford Site. Research indicates that some of the contaminant species …
Flap Gate, Charles M. Burt
Flap Gate, Charles M. Burt
BioResource and Agricultural Engineering
ITRC began to investigate early designs of Flap Gates in 1992. The gates originated in The Netherlands in the 1940s, but no design criteria were available. ITRC developed an Excel design program that uses a combination of theory and coefficients from field results – and has continued to improve the program almost every year. District staff at Turlock ID and Chowchilla WD provided valuable feedback with early models. This report includes information on design, installation, maintenance, water conservation and efficiency, cost, use, and benefits.
ITRC Flap Gates are typically used with a concrete counterweight in situations where the gates are …
General Irrigation Consumer Bill Of Rights, Charles M. Burt
General Irrigation Consumer Bill Of Rights, Charles M. Burt
BioResource and Agricultural Engineering
Cal Poly ITRC, together with various irrigation industry leaders and the Irrigation Dealers Association of California, plus with assistance from the California Energy Commission and Pacific Gas & Electric Co., developed the Irrigation Consumer Bill of Rights (ICBR) in 1994.
Respiration Of Metal (Hydr)Oxides By Shewanella And Geobacter: A Key Role For Multihaem C-Type Cytochromes, Liang Shi, Thomas C. Squier, John M. Zachara, James K. Fredrickson
Respiration Of Metal (Hydr)Oxides By Shewanella And Geobacter: A Key Role For Multihaem C-Type Cytochromes, Liang Shi, Thomas C. Squier, John M. Zachara, James K. Fredrickson
US Department of Energy Publications
Dissimilatory reduction of metal (e.g. Fe, Mn) (hydr)oxides represents a challenge for microorganisms, as their cell envelopes are impermeable to metal (hydr)oxides that are poorly soluble in water. To overcome this physical barrier, the Gram-negative bacteria Shewanella oneidensis MR-1 and Geobacter sulfurreducens have developed electron transfer (ET) strategies that require multihaem c-type cytochromes (c-Cyts). In S. oneidensis MR-1, multihaem c-Cyts CymA and MtrA are believed to transfer electrons from the inner membrane quinone/quinol pool through the periplasm to the outer membrane. The type II secretion system of S. oneidensis MR-1 has been implicated in the reduction of metal …
Temperature Effect On Tert-Butyl Alcohol (Tba) Biodegradation Kinetics In Hyporheic Zone Soils, M. H. Greenwood, Ronald C. Sims, J. E. Mclean, W. J. Doucette
Temperature Effect On Tert-Butyl Alcohol (Tba) Biodegradation Kinetics In Hyporheic Zone Soils, M. H. Greenwood, Ronald C. Sims, J. E. Mclean, W. J. Doucette
Biological Engineering Faculty Publications
Background
Remediation of tert-butyl alcohol (TBA) in subsurface waters should be taken into consideration at reformulated gasoline contaminated sites since it is a biodegradation intermediate of methyl tert-butyl ether (MTBE), ethyl tert-butyl ether (ETBE), and tert-butyl formate (TBF). The effect of temperature on TBA biodegradation has not been not been published in the literature.
Methods
Biodegradation of [U 14C] TBA was determined using hyporheic zone soil microcosms.
Results
First order mineralization rate constants of TBA at 5°C, 15°C and 25°C were 7.84 ± 0.14 × 10-3, 9.07 ± 0.09 × 10-3, and 15.3 ± 0.3 …
Intellectual Property Management In Health And Agricultural Innovation: Executive Guide, Anatole Krattiger, Richard T. Mahoney, Lita Nelsen, Jennifer A. Thomson, Alan B. Bennett, Kanikaram Satyanarayana, Gregory D. Graff, Carlos Fernandez, Stanley Kowalski
Intellectual Property Management In Health And Agricultural Innovation: Executive Guide, Anatole Krattiger, Richard T. Mahoney, Lita Nelsen, Jennifer A. Thomson, Alan B. Bennett, Kanikaram Satyanarayana, Gregory D. Graff, Carlos Fernandez, Stanley Kowalski
Law Faculty Scholarship
Prepared by and for policy-makers, leaders of public sector research establishments, technology transfer professionals, licensing executives, and scientists, this online resource offers up-to-date information and strategies for utilizing the power of both intellectual property and the public domain. Emphasis is placed on advancing innovation in health and agriculture, though many of the principles outlined here are broadly applicable across technology fields. Eschewing ideological debates and general proclamations, the authors always keep their eye on the practical side of IP management. The site is based on a comprehensive Handbook and Executive Guide that provide substantive discussions and analysis of the opportunities …
Intellectual Property Management In Health And Agricultural Innovation: A Handbook Of Best Practices, Vol. 1, Anatole Krattiger, Richard T. Mahoney, Lita Nelsen, Jennifer A. Thomson, Alan B. Bennett, Kanikaram Satyanarayana, Gregory D. Graff, Carlos Fernandez, Stanley Kowalski
Intellectual Property Management In Health And Agricultural Innovation: A Handbook Of Best Practices, Vol. 1, Anatole Krattiger, Richard T. Mahoney, Lita Nelsen, Jennifer A. Thomson, Alan B. Bennett, Kanikaram Satyanarayana, Gregory D. Graff, Carlos Fernandez, Stanley Kowalski
Law Faculty Scholarship
Prepared by and for policy-makers, leaders of public sector research establishments, technology transfer professionals, licensing executives, and scientists, this online resource offers up-to-date information and strategies for utilizing the power of both intellectual property and the public domain. Emphasis is placed on advancing innovation in health and agriculture, though many of the principles outlined here are broadly applicable across technology fields. Eschewing ideological debates and general proclamations, the authors always keep their eye on the practical side of IP management. The site is based on a comprehensive Handbook and Executive Guide that provide substantive discussions and analysis of the opportunities …
Rational Risk/Benefit Analysis Of Genetically Modified Crops, Stanley P. Kowalski
Rational Risk/Benefit Analysis Of Genetically Modified Crops, Stanley P. Kowalski
Law Faculty Scholarship
Safety concerns over the use of molecular biotechnology in the improvement of crops has generated substantial, heated and confusing debates, often driven by ideology and hysterics. Modification of crops is not new, and biotechnology (in its broadest sense) has been used for over a century to accelerate the development of new crops for food, feed and fibre, so as to meet the demands of a growing global community. The introduction of crops developed via molecular biotechnology [Genetically Modified Crops (GMCs)] represents the latest step in this inexorable innovative progression of technology. However, misinformed concern has led to a broad embrace …
Economic Analysis Of Cellulase Production Methods For Bio-Ethanol, Jun Zhuang, Mary A. Marchant, Sue E. Nokes, Herbert J. Strobel
Economic Analysis Of Cellulase Production Methods For Bio-Ethanol, Jun Zhuang, Mary A. Marchant, Sue E. Nokes, Herbert J. Strobel
Biosystems and Agricultural Engineering Faculty Publications
The cost of cellulase enzymes has limited the feasibility of producing ethanol from fibrous biomass. Traditional submerged fermentation (SmF) was compared to an alternative method of producing cellulase, solid state cultivation (SSC). Results from an economic analysis indicated that the unit costs for cellulase enzyme production were $15.67 (The prices are all 2004 prices in this article, except otherwise stated. We deflated newer prices to 2004 prices using a deflation factor 0.9 per year and inflated older prices to 2004 prices using an inflation factor 1.1.) per kilogram ($/kg) and $40.36/kg, for the SSC and SmF methods, respectively, while the …
Convergence Of Peridynamics To Classical Elasticity Theory, S. A. Silling, R. B. Lehoucq
Convergence Of Peridynamics To Classical Elasticity Theory, S. A. Silling, R. B. Lehoucq
US Department of Energy Publications
The peridynamic model of solid mechanics is a nonlocal theory containing a length scale. It is based on direct interactions between points in a continuum separated from each other by a finite distance. The maximum interaction distance provides a length scale for the material model. This paper addresses the question of whether the peridynamic model for an elastic material reproduces the classical local model as this length scale goes to zero. We show that if the motion, constitutive model, and any nonhomogeneities are sufficiently smooth, then the peridynamic stress tensor converges in this limit to a Piola-Kirchhoff stress tensor that …
Peridynamic States And Constitutive Modeling, S. A. Silling, M. Epton, O. Weckner, J. Xu, E. Askari
Peridynamic States And Constitutive Modeling, S. A. Silling, M. Epton, O. Weckner, J. Xu, E. Askari
US Department of Energy Publications
A generalization of the original peridynamic framework for solid mechanics is proposed. This generalization permits the response of a material at a point to depend collectively on the deformation of all bonds connected to the point. This extends the types of material response that can be reproduced by peridynamic theory to include an explicit dependence on such collectively determined quantities as volume change or shear angle. To accomplish this generalization, a mathematical object called a deformation state is defined, a function that maps any bond onto its image under the deformation. A similar object called a force state is defined, …
Efficiency Of Gene Silencing In Arabidopsis: Direct Inverted Repeats Vs. Transitive Rnai Vectors, Sergei A. Filichkin, Stephen P. Difazio, Amy M. Brunner, John M. Davis, Zamin K. Yang, Udaya C. Kalluri, Renee S. Arias, Elizabeth Etherington, Gerald A. Tuskan, Steven H. Strauss
Efficiency Of Gene Silencing In Arabidopsis: Direct Inverted Repeats Vs. Transitive Rnai Vectors, Sergei A. Filichkin, Stephen P. Difazio, Amy M. Brunner, John M. Davis, Zamin K. Yang, Udaya C. Kalluri, Renee S. Arias, Elizabeth Etherington, Gerald A. Tuskan, Steven H. Strauss
US Department of Energy Publications
We investigated the efficiency of RNA interference (RNAi) in Arabidopsis using transitive and homologous inverted repeat (hIR) vectors. hIR constructs carry self-complementary intron-spliced fragments of the target gene whereas transitive vectors have the target sequence fragment adjacent to an intron-spliced, inverted repeat of heterologous origin. Both transitive and hIR constructs facilitated specific and heritable silencing in the three genes studied (AP1 , ETTIN and TTG1 ). Both types of vectors produced a phenotypic series that phenocopied reduction of function mutants for the respective target gene. The hIR yielded up to fourfold higher proportions of events with strongly manifested reduction …
Phosphate Imposed Limitations On Biological Reduction And Alteration Of Ferrihydrite, Thomas Borch, Yoko Masue, Ravi K. Kukkadapu, Scott Fendorf
Phosphate Imposed Limitations On Biological Reduction And Alteration Of Ferrihydrite, Thomas Borch, Yoko Masue, Ravi K. Kukkadapu, Scott Fendorf
US Department of Energy Publications
Biogeochemical transformation (inclusive of dissolution) of iron (hydr)oxides resulting from dissimilatory reduction has a pronounced impact on the fate and transport of nutrients and contaminants in subsurface environments. Despite the reactivity noted for pristine (unreacted) minerals, iron (hydr)oxides within native environments will likely have a different reactivity owing in part to changes in surface composition. Accordingly, here we explore the impact of surface modifications induced by phosphate adsorption on ferrihydrite reduction by Shewanella putrefaciens under static and advective flow conditions. Alterations in surface reactivity induced by phosphate changes the extent, decreasing Fe(III) reduction nearly linearly with increasing P surface coverage, …