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2014

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Full-Text Articles in Bioresource and Agricultural Engineering

Feedlot Manure Management Considerations Including Anaerobic Digestion Potential And Mineral Retention, Andrea K. Watson Dec 2014

Feedlot Manure Management Considerations Including Anaerobic Digestion Potential And Mineral Retention, Andrea K. Watson

Department of Animal Science: Dissertations, Theses, and Student Research

Three anaerobic digestion trials were conducted to evaluate methane production from feedlot manure. As organic matter content of manure decreased, degradation of manure and methane production also linearly decreased (P ≤ 0.02). Quality, or organic matter content of manure, had a greater impact on anaerobic digestion than diet (with and without distillers grains) fed to cattle. Minerals are concentrated in effluent removed from anaerobic digesters.

Knowing mineral retention within cattle allows for more accurate calculation of mineral excretion in manure. Three serial slaughter trials measured mineral retention in growing and finishing cattle. During a growing phase, beef cattle gaining …


Methane Production By A Packed-Bed Anaerobic Digester Fed Dairy Barn Flush Water, Sean Richard Thomson Dec 2014

Methane Production By A Packed-Bed Anaerobic Digester Fed Dairy Barn Flush Water, Sean Richard Thomson

Master's Theses

Packed-bed digesters are an alternative to covered lagoon digesters for methane production and anaerobic treatment of dilute wastewaters such as dairy barn flush water. The physical media of packed-beds retain biofilms, often allowing increased treatment rates. Previous studies have evaluated several types of media for digestion of dilute wastewaters, but cost and media fouling have setback commercial development. A major operational cost has been effluent recirculation pumping.

In the present effort, a novel approach to anaerobic digestion of flush dairy water was developed at pilot-scale: broken walnut shells were used as a low-cost packed-bed medium and effluent recirculation was replaced …


United States Patent Application Publication, John H. Posselius, Christopher A. Foster, Santosh Pitla, Scott A. Shearer, Joe D. Luck, Michael P. Sama, Rodrigo S. Zandonadi Nov 2014

United States Patent Application Publication, John H. Posselius, Christopher A. Foster, Santosh Pitla, Scott A. Shearer, Joe D. Luck, Michael P. Sama, Rodrigo S. Zandonadi

Department of Biological Systems Engineering: Papers and Publications

A multiple robot control architecture including a plurality of robotic agricultural machines including a first and second robotic agricultural machine. Each robotic agricultural machine including at least one controller configured to implement a plurality of finite state machines Within an individual robot control architecture (IRCA) and a global information module (GIM) communicatively coupled to the IRCA. The GIMs of the first and second robotic agricultural machines being configured to cooperate to cause said first robotic agricultural machine and said second agricultural machine to perform at least one agricultural task.


Nutrient Runoff Following Swine Manure Application, Seth J. Lamb Nov 2014

Nutrient Runoff Following Swine Manure Application, Seth J. Lamb

Department of Environmental Engineering: Theses and Student Research

A field study was completed to compare the effects of land application methods, swine growth stage, and varying flow rates following the application of varying amounts of swine manure to 0.75-m by 2.00-m long plots. Three different manure sources were used to represent key different growth stages including growers, finishers, and sows and gilts. The different swine manures were applied in May and Jun 2011 to meet a 1 year nitrogen (N) requirement for corn. Three different land application methods were used to apply the swine manure, broadcast, incorporation, and injection. Runoff water quality was measured during three 30 minute …


Reply To ‘Co2 Emissions From Crop Residue-Derived Biofuels’, Adam Liska, Haishun Yang, Matthew P. Pelton, Andrew E. Suyker Oct 2014

Reply To ‘Co2 Emissions From Crop Residue-Derived Biofuels’, Adam Liska, Haishun Yang, Matthew P. Pelton, Andrew E. Suyker

Adam Liska Papers

The soil organic carbon (SOC) model that we used was parameterized with data from arable land under normal farming conditions in North America, Europe, Africa and Asia, but the equation is insensitive to changes in tillage, soil texture and moisture. The model has reasonable accuracy, however, in predicting changes in SOC, residue remaining and CO2 emissions from initial SOC, carbon inputs from residue, and daily temperature; the shoot-to-root ratio used in the geospatial simulation was 0.29 (that is, root carbon is 29% of total aboveground carbon), which did not underestimate carbon input to soil (Supplementary Figure 2 in Ref. …


Soil Carbon Accumulation Under Switchgrass Barriers, Humberto Blanco-Canqui, John E. Gilley, Dean E. Eisenhauer, Paul J. Jasa, Alan L. Boldt Oct 2014

Soil Carbon Accumulation Under Switchgrass Barriers, Humberto Blanco-Canqui, John E. Gilley, Dean E. Eisenhauer, Paul J. Jasa, Alan L. Boldt

Department of Biological Systems Engineering: Papers and Publications

The benefits of grass barriers or hedges for reducing offsite transport of non-point-source water pollutants from croplands are well recognized, but their ancillary benefits on soil properties have received less attention. We studied the 15-yr cumulative effects of narrow and perennial switchgrass (Panicum virgatum L.) barriers on soil organic C (SOC), total N, particulate organic matter (POM), and associated soil structural properties as compared with the cropped area on an Aksarben silty clay loam (fine, smectitic, mesic Typic Argiudoll) with 5.4% slope in eastern Nebraska. Five switchgrass barriers were established in 1998 at ~38-m intervals parallel to the crop rows …


Development Of An Interactive Water Management Role‐Playing Game As An Educational Tool Representing The Central Nebraska Platte River Valley Social‐Economic‐Ecological System, Victoria Chraibi, Nathan Rossman, Anil Giri, Noelle Hart, Zion Schell Oct 2014

Development Of An Interactive Water Management Role‐Playing Game As An Educational Tool Representing The Central Nebraska Platte River Valley Social‐Economic‐Ecological System, Victoria Chraibi, Nathan Rossman, Anil Giri, Noelle Hart, Zion Schell

DBER Speaker Series

As members of the National Science Foundation's Integrative Graduate Education and Research Traineeship (IGERT), we have gained valuable experience abroad in Europe learning about and participating in research that utilizes serious role‐playing games representing a watershed with its land use and river system. The focus of this research is on the development of such a game representing the central Platte River valley and its dynamics according to the activities played by the players and uncertain streamflow inputs. The game is adapted specifically from two other games, one called Wat‐A‐Game, developed at the French public institutes IRSTEA and CIRAD (https://sites.google.com/site/waghistory/ …


Agricultural Industry Advanced Vehicle Technology: Benchmark Study For Reduction In Petroleum Use, Roger M. Hoy, Rodney Rohrer, Adam Liska, Joe D. Luck, Loren Isom, Deepak R. Keshwani Sep 2014

Agricultural Industry Advanced Vehicle Technology: Benchmark Study For Reduction In Petroleum Use, Roger M. Hoy, Rodney Rohrer, Adam Liska, Joe D. Luck, Loren Isom, Deepak R. Keshwani

Adam Liska Papers

Diesel use on farms in the United States has remained relatively constant since 1985, decreasing slightly in 2009, which may be attributed to price increases and the economic recession (Figure 1). During this time, the United States’ harvested area also has remained relatively constant at roughly 300 million acres. In 2010, farm diesel use was 5.4% of the total United States diesel use. Crops accounting for an estimated 65% of United States farm diesel use include corn, soybean, wheat, hay, and alfalfa, respectively, based on harvested crop area and a recent analysis of estimated fuel use by crop (Figure 2).1 …


Infiltration And Runoff Parameters For Tilled And No-Till Row Crops, Andrew J. Volkmer Aug 2014

Infiltration And Runoff Parameters For Tilled And No-Till Row Crops, Andrew J. Volkmer

Department of Biological Systems Engineering: Dissertations, Theses, and Student Research

Infiltration and runoff are hydrologic processes that effect the amount of water available to plants, for groundwater recharge, and for stream flow. No-till planting (NT) is a management practice used to reduce soil erosion, increase water infiltration, and reduce soil water evaporation, and can have great impact on infiltration and runoff. An investigation was conducted to determine the impact of NT on infiltration and runoff when compared to tilled conditions.

Runoff and precipitation data was gathered from sites at Fillmore County, NE, Phelps County, NE, the USDA-ARS North Appalachian Experimental Watersheds (NAEW) near Coshocton, OH, and the Lennoxville Research Station …


Estimating Potential Water Pump Reductions Based On Soil Water Content, Geospatial Data Layers, And Variable Rate Irrigation (Vri) Pivot Control Resolution, Keith A. Miller Aug 2014

Estimating Potential Water Pump Reductions Based On Soil Water Content, Geospatial Data Layers, And Variable Rate Irrigation (Vri) Pivot Control Resolution, Keith A. Miller

Department of Biological Systems Engineering: Dissertations, Theses, and Student Research

Increasing concern for sustainable water use has the agriculture industry working toward higher efficiency use of irrigation water. The average irrigation water use efficiency throughout the United States is 45%, which is extremely poor. Advancements in crop management have continued to allow producers to know more about the conditions in their field from nutrient management and pest control, to understanding yield spatially.

Recent mechanical advancements have improved the capabilities of center pivot irrigation systems to water various depths throughout the field. This technology is known as variable rate irrigation (VRI). With VRI comes a whole new strategy for irrigation. Advancements …


Emission Of Volatile Organic Compounds After Land Application Of Cattle Manure, Bryan Woodbury, John E. Gilley, David B. Parker, David B. Marx, Daniel N. Miller, Roger A. Eigenberg Jul 2014

Emission Of Volatile Organic Compounds After Land Application Of Cattle Manure, Bryan Woodbury, John E. Gilley, David B. Parker, David B. Marx, Daniel N. Miller, Roger A. Eigenberg

Department of Biological Systems Engineering: Papers and Publications

Beef cattle manure can serve as a valuable source of nutrients for crop production. However, emissions of volatile organic compounds (VOCs) after land application may pose an odor nuisance to downwind populations. This study was conducted to evaluate the effects of land application method, diet, soil moisture content, and time since manure application on VOC emissions. Manure was collected from feedlot pens where cattle were fed diets containing 0, 10, or 30% wet distillers grains with solubles (WDGS). Land application methods included surface-applying manure (i.e., no-tillage) or incorporating manure using disk tillage. The effects of soil moisture content on VOC …


Sustainable Development Of Biogas Resources In East Africa Using Transdisciplinary Design Models, Christopher M. Limiac Jul 2014

Sustainable Development Of Biogas Resources In East Africa Using Transdisciplinary Design Models, Christopher M. Limiac

Open Access Theses

Installation of anaerobic digestion systems in urban developing communities has the potential to address multiple problems related to energy and sanitation in a combined systems approach. Anaerobic digesters are used to generate a relatively clean and rich source of energy in the form of biogas to address energy poverty issues. The design resources available for anaerobic digestion projects must be improved to provide greater technical support and project transparency. In order to develop holistic solutions to grand challenges like energy poverty, engineers must be prepared to operate with the complexities of diverse environments. Globalization and the improvement of transdisciplinary project …


The Applicability Of Vri For Managing Variability In Infiltration Capacity And Plant-Available Water: A Preliminary Discussion And Gis Study, Tsz Him Lo, Luciano Mateos, Derek M. Heeren, Joe D. Luck Jul 2014

The Applicability Of Vri For Managing Variability In Infiltration Capacity And Plant-Available Water: A Preliminary Discussion And Gis Study, Tsz Him Lo, Luciano Mateos, Derek M. Heeren, Joe D. Luck

Department of Biological Systems Engineering: Papers and Publications

Although variable rate irrigation (VRI) has been researched and marketed for a number of years, research that quantifies the magnitude of VRI’s potential benefits and that are translatable to unmonitored fields is lacking. The potential reduction in seasonal irrigation is proposed as the criterion for beginning to evaluate the use of VRI to improve agricultural water management when infiltration capacity and plant-available water are spatially variable inside a field. An initial geographic information system study using publicly available geospatial data was conducted to examine soil and topographic properties associated with such variability within 1100 center pivots across 11 counties. The …


Finite Element Modeling Of Long-Term Phosphorus Leaching Through Macropores In The Ozark Ecoregion, Ryan P. Freiberger, Derek M. Heeren, Garey A. Fox, Chad J. Penn, Dean E. Eisenhauer Jul 2014

Finite Element Modeling Of Long-Term Phosphorus Leaching Through Macropores In The Ozark Ecoregion, Ryan P. Freiberger, Derek M. Heeren, Garey A. Fox, Chad J. Penn, Dean E. Eisenhauer

Department of Biological Systems Engineering: Papers and Publications

Phosphorus (P) is a critical nutrient for plant growth in agriculture, but is also responsible for surface water enrichment that leads to toxic algal growth. While P loading to surface waters has traditionally been thought to occur from surface runoff, contributions from subsurface transport can also be significant. While P transport through many soil types is well-documented, the presence of highly conductive gravel outcrops and macropore networks can have a significant, yet poorly-documented effect on P movement to the aquifer. Floodplain soils in the Ozark ecoregion generally contain coarse chert gravel layers that exhibit macropore behavior. Previous research has evaluated …


Combining Site Specific Data With Geospatial Analysis To Identify Variable Rate Irrigation Opportunities In Irrigated Agricultural Fields., K. A. Miller, T. H. Lo, J. D. Luck, D. M. Heeren Jul 2014

Combining Site Specific Data With Geospatial Analysis To Identify Variable Rate Irrigation Opportunities In Irrigated Agricultural Fields., K. A. Miller, T. H. Lo, J. D. Luck, D. M. Heeren

Department of Biological Systems Engineering: Papers and Publications

Increasing demand for improving irrigation water use efficiency (IWUE) across irrigated croplands has producers looking for new opportunities to conserve water and maintain their crop production levels. Variable Rate Irrigation (VRI) technology may provide opportunities to enhance IWUE, but as with site-specific crop management of other crop inputs, all fields may not benefit from an approach which utilizes VRI. To consider the potential benefits of VRI, it will be necessary to examine multiple site-specific spatial data layers (e.g., crop yield, soil type/texture, and terrain) as well as the current conditions within a field using geospatial analysis techniques.

The goal of …


Investigating Antibiotic Resistance Levels Of Salmonella Internalized In Lettuce Leaves, Jason B. Thomas May 2014

Investigating Antibiotic Resistance Levels Of Salmonella Internalized In Lettuce Leaves, Jason B. Thomas

McNair Scholars Research Journal

Contamination of food crops by the human pathogen Salmonella is a food safety threat worldwide. Though using treated wastewater for irrigation is a sustainable practice, it may introduce trace levels of Salmonella that may contaminate food crops. Salmonella could develop resistance to antibiotics present in wastewater. The overall goal of the project is to increase the understanding of the public health risk associated with the use of treated wastewater to irrigate food crops. The objective of this particular study is to determine the antibiotic resistance level of Salmonella internalized in lettuce leaves. In this experiment, thirty-six plants of the lettuce …


A Hydrological Analysis Of Switchgrass Land Cover In East Tennessee, Jordan Avery Hayes May 2014

A Hydrological Analysis Of Switchgrass Land Cover In East Tennessee, Jordan Avery Hayes

Masters Theses

Energy needs and the recent installation of a cellulosic biofuel plant in Vonore, TN have created a demand for switchgrass in East Tennessee. Switchgrass has many strengths such as erosion protection, nutrient removal, and runoff mitigation. The Soil and Water Assessment Tool (SWAT) was selected to model the impact of transitioning traditional crops into switchgrass land cover. Field data was needed to properly calibrate the SWAT model for East Tennessee. The National Resources Conservation Service (NRCS) curve number (CN) was needed for runoff calibrations. This value was determined by both standard NRCS methods as well as an asymptotic method. The …


Damage Mapping Of Powdery Mildew In Winter Wheat With High-Resolution Satellite Image, Lin Yuan, Jingcheng Zhang, Yeyin Shi, Chenwei Nie, Liguang Wei, Jihua Wang Apr 2014

Damage Mapping Of Powdery Mildew In Winter Wheat With High-Resolution Satellite Image, Lin Yuan, Jingcheng Zhang, Yeyin Shi, Chenwei Nie, Liguang Wei, Jihua Wang

Department of Biological Systems Engineering: Papers and Publications

Powdery mildew, caused by the fungus Blumeria graminis, is a major winter wheat disease in China. Accurate delineation of powdery mildew infestations is necessary for site-specific disease management. In this study, high-resolution multispectral imagery of a 25 km2 typical outbreak site in Shaanxi, China, taken by a newly-launched satellite, SPOT-6, was analyzed for mapping powdery mildew disease. Two regions with high representation were selected for conducting a field survey of powdery mildew. Three supervised classification methods—artificial neural network, mahalanobis distance, and maximum likelihood classifier—were implemented and compared for their performance on disease detection. The accuracy assessment showed that the ANN …


Biofuels From Crop Residue Can Reduce Soil Carbon And Increase Co2 Emissions, Adam J. Liska, Haishun Yang, Maribeth Milner, Steve Goddard, Humberto Blanco-Canqui, Matthew P. Pelton, Xiao X. Fang, Haitao Zhu, Andrew E. Suyker Apr 2014

Biofuels From Crop Residue Can Reduce Soil Carbon And Increase Co2 Emissions, Adam J. Liska, Haishun Yang, Maribeth Milner, Steve Goddard, Humberto Blanco-Canqui, Matthew P. Pelton, Xiao X. Fang, Haitao Zhu, Andrew E. Suyker

Adam Liska Papers

Removal of corn residue for biofuels can decrease soil organic carbon(SOC) and increase CO2 emissions because residue C in biofuels is oxidized to CO2 at a faster rate than when added to soil. Net CO2 emissions from residue removal are not adequately characterized in biofuel life cycle assessment (LCA). Here we used a model to estimate CO2 emissions from corn residue removal across the US Corn Belt at 580 million geospatial cells. To test the SOC model, we compared estimated daily CO2 emissions from corn residue and soil with CO2 emissions measured using eddy covariance, …


Identification Of Wheat Varieties With A Parallel-Plate Capacitance Sensor Using Fisher’S Linear Discriminant Analysis, C. V. K. Kandala, George Meyer, N. Puppala, V. Settaluri, R. S. Reddy Feb 2014

Identification Of Wheat Varieties With A Parallel-Plate Capacitance Sensor Using Fisher’S Linear Discriminant Analysis, C. V. K. Kandala, George Meyer, N. Puppala, V. Settaluri, R. S. Reddy

Department of Biological Systems Engineering: Papers and Publications

Fisher’s linear discriminant (FLD) models for wheat variety classification were developed and validated. The inputs to the FLD models were the capacitance (𝐶), impedance (𝑍), and phase angle (𝜃), measured at two frequencies. Classification of wheat varieties was obtained as output of the FLDmodels.𝑍and 𝜃of a parallel-plate capacitance system,holding thewheat samples, weremeasured using an impedance meter, and the 𝐶 value was computed.The best model developed classified the wheat varieties, with accuracy of 95.4%, over the six wheat varieties tested.This method is simple, rapid, and nondestructive and would be useful for the breeders and the peanut industry.


Berm Method For Quantification Of Infiltration At The Plot Scale In High Conductivity Soils, Derek M. Heeren, Garey A. Fox, Daniel E. Storm Feb 2014

Berm Method For Quantification Of Infiltration At The Plot Scale In High Conductivity Soils, Derek M. Heeren, Garey A. Fox, Daniel E. Storm

Department of Biological Systems Engineering: Papers and Publications

Measuring infiltration at the plot scale is difficult, especially for high hydraulic conductivity soils. At the plot scale, the infiltration rate is usually calculated by comparing surface runoff to rainfall. Direct measurement of infiltration beyond the point scale is typically limited to locations where land forming (e.g., infiltration pond) has been performed or fields with basin irrigation systems. The standard method for field measurement of point-scale infiltration is the double ring infiltrometer, which is limited in size (typically 30 cm diameter). In this research, a new method is proposed that uses a temporary berm constructed of a water-filled 15-cm diameter …


Application Of Isothermal Calorimetry To Phosphorus Sorption Onto Soils In A Flow-Through System, Chad Penn, Derek M. Heeren, Garey A. Fox, Ajay Kumar Jan 2014

Application Of Isothermal Calorimetry To Phosphorus Sorption Onto Soils In A Flow-Through System, Chad Penn, Derek M. Heeren, Garey A. Fox, Ajay Kumar

Department of Biological Systems Engineering: Papers and Publications

The degree, mechanisms, and kinetics of phosphorus (P) sorption onto soils can have a significant influence on leaching losses of P from soil. The objectives of this study were to measure the impact of retention time (RT) on P sorption in a flow-through system intended to simulate downward movement of a P solution through two different riparian soils, and determine if isothermal titration calorimetry (ITC) can provide useful information reflective of flow-through results. Topsoil from two riparian/alluvial sites (Barren Fork and Clear Creek) was sampled and characterized for P concentrations and parameters related to P sorption. Flow-through P sorption experiments …


2014 Nebraska Groundwater Quality Monitoring Report Jan 2014

2014 Nebraska Groundwater Quality Monitoring Report

Nebraska Department of Environmental Quality: Reports

The 2001 Nebraska Legislature passed LB329 (Neb. Rev. Stat. §46-1304) which, in part, directed the Nebraska Department of Environmental Quality (NDEQ) to report on groundwater quality monitoring in Nebraska. Reports have been issued annually since December 2001. The text of the statute applicable to this report follows: “The Department of Environmental Quality shall prepare a report outlining the extent of ground water quality monitoring conducted by natural resources districts during the preceding calendar year. The department shall analyze the data collected for the purpose of determining whether or not ground water quality is degrading or improving and shall present the …


Implementing Green Roofs On Movie Theaters And Shopping Centers: Business Cases In Profitable Sustainability, Ryan J. Miller Jan 2014

Implementing Green Roofs On Movie Theaters And Shopping Centers: Business Cases In Profitable Sustainability, Ryan J. Miller

Pomona Senior Theses

This thesis presents the business case for installing green roofs on movie theaters and shopping centers. These businesses can then derive increased profits from the environmental benefits of reduced energy use and increased stormwater retention. After presenting the basic design and benefits of a green roof, the thesis develops stand-alone business plans for a movie theater and shopping center. The author finds that green roofs are a profitable sustainability solution for the commercial enterprise.


2014 Nebraska Water Monitoring Programs Report, Marty Link, Ryan Chapman Jan 2014

2014 Nebraska Water Monitoring Programs Report, Marty Link, Ryan Chapman

Nebraska Department of Environmental Quality: Reports

The Nebraska Department of Environmental Quality (NDEQ) is charged with monitoring, assessing, and to the extent possible, managing the state’s water resources. The purpose of this work is to protect and maintain high quality water and encourage or execute activities to improve poor water quality. Monitoring is done on nearly 17,000 miles of flowing rivers and streams, more than 134,000 acres of surface water in lakes and reservoirs, as well as the vast storage of groundwater in Nebraska’s aquifers.


Flash Hydrolysis Of Microalgae Biomass For Biofuels Intermediates Production, Protein Extraction, And Nutrients Recycle, Jose Luis Garcia Moscoso Jan 2014

Flash Hydrolysis Of Microalgae Biomass For Biofuels Intermediates Production, Protein Extraction, And Nutrients Recycle, Jose Luis Garcia Moscoso

Civil & Environmental Engineering Theses & Dissertations

Microalgae have shown much higher growth rates and productivity when compared to conventional agricultural crops, aquatic plants and tree species, requiring much less land area than other biodiesel feedstock. To harness that potential the hydrothermal liquefaction of algae biomass was studied and a new process called "Flash Hydrolysis" was developed to use water under subcritical conditions, this process capitalizes on the difference in reaction kinetics of algae polymeric components and fractionates proteins in liquid phase in seconds of residence time.

The main objectives for this study are: Analyze the effect of temperature in FH process to maximize the extraction of …


Water-Stable Three-Dimensional Ultrafine Fibrous Scaffolds From Keratin For Cartilage Tissue Engineering, Helan Xu, Shaobo Cai, Lan Xu, Yiqi Yang Jan 2014

Water-Stable Three-Dimensional Ultrafine Fibrous Scaffolds From Keratin For Cartilage Tissue Engineering, Helan Xu, Shaobo Cai, Lan Xu, Yiqi Yang

Department of Biological Systems Engineering: Papers and Publications

Intrinsically water-stable scaffolds composed of ultrafine keratin fibers oriented randomly and evenly in three dimensions were electrospun for cartilage tissue engineering. Keratin has been recognized as a biomaterial that could substantially support the growth and development of multiple cell lines. Besides, three-dimensional (3D) ultrafine fibrous structures were preferred in tissue engineering due to their structural similarity to native extracellular matrices in soft tissues. Recently, we have developed a nontraditional approach to developing 3D fibrous scaffolds from alcohol-soluble corn protein, zein, and verified their structural advantages in tissue engineering. However, keratin with highly cross-linked molecular structures could not be readily dissolved …


Intrinsically Water-Stable Keratin Nanoparticles And Their In Vivo Biodistribution For Targeted Delivery, Helan Xu, Zhen Shi, Narendra Reddy, Yiqi Yang Jan 2014

Intrinsically Water-Stable Keratin Nanoparticles And Their In Vivo Biodistribution For Targeted Delivery, Helan Xu, Zhen Shi, Narendra Reddy, Yiqi Yang

Department of Biological Systems Engineering: Papers and Publications

Highly water-stable nanoparticles of around 70 nm and capable of distributing with high uptake in certain organs of mice were developed from feather keratin. Nanoparticles could provide novel veterinary diagnostics and therapeutics to boost efficiency in identification and treatment of livestock diseases to improve protein supply and ensure safety and quality of food. Nanoparticles could penetrate easily into cells and small capillaries, surpass detection of the immune system, and reach targeted organs because of their nanoscale sizes. Proteins with positive and negative charges and hydrophobic domains enable loading of various types of drugs and, hence, are advantageous over synthetic polymers …


Surface-Enhanced Raman Spectral Biomarkers Correlate With Ankle Brachial Index And Characterize Leg Muscle Biochemical Composition Of Patients With Peripheral Arterial Disease, Kim Cluff, Abby M. Kelly, Panagiotis Koutakis, Xiang N. He, Xi Huang, Yongfeng Lu, Iraklis I. Pipinos, George P. Casale, Jeyamkondan Subbiah Jan 2014

Surface-Enhanced Raman Spectral Biomarkers Correlate With Ankle Brachial Index And Characterize Leg Muscle Biochemical Composition Of Patients With Peripheral Arterial Disease, Kim Cluff, Abby M. Kelly, Panagiotis Koutakis, Xiang N. He, Xi Huang, Yongfeng Lu, Iraklis I. Pipinos, George P. Casale, Jeyamkondan Subbiah

Department of Biological Systems Engineering: Papers and Publications

Peripheral arterial disease (PAD) is characterized by atherosclerotic blockages of the arteries supplying the lower extremities, which cause a progressive accumulation of ischemic injury to the skeletal muscles of the lower limbs. This injury includes altered metabolic processes, damaged organelles, and compromised bioenergetics in the affected muscles. The objective of this study was to explore the association of Raman spectral signatures of muscle biochemistry with the severity of atherosclerosis in the legs as determined by the Ankle Brachial Index (ABI) and clinical presentation. We collected muscle biopsies from the gastrocnemius (calf muscle) of five patients with clinically diagnosed claudication, five …


Experimental Tools To Study Molecular Recognition Within The Nanoparticle Corona, Markita P. Landry, Sebastian Kruss, Justin T. Nelson, Gili Bisker, Nicole M. Iverson, Nigel F. Reuel, Michael S. Strano Jan 2014

Experimental Tools To Study Molecular Recognition Within The Nanoparticle Corona, Markita P. Landry, Sebastian Kruss, Justin T. Nelson, Gili Bisker, Nicole M. Iverson, Nigel F. Reuel, Michael S. Strano

Department of Biological Systems Engineering: Papers and Publications

Advancements in optical nanosensor development have enabled the design of sensors using synthetic molecular recognition elements through a recently developed method called Corona Phase Molecular Recognition (CoPhMoRe). The synthetic sensors resulting from these design principles are highly selective for specific analytes, and demonstrate remarkable stability for use under a variety of conditions. An essential element of nanosensor development hinges on the ability to understand the interface between nanoparticles and the associated corona phase surrounding the nanosensor, an environment outside of the range of traditional characterization tools, such as NMR. This review discusses the need for new strategies and instrumentation to …