Biomedical Engineering and Bioengineering Commons^™

Spatiotemporal Changes In Nuclear Strain Measured By Traction Force Microscopy, Ryan D. Watts, Corey Neu, Jonathan Henderson

The Summer Undergraduate Research Fellowship (SURF) Symposium

The knowledge of how cells interact with and sense their surroundings is missing the key components of time dependency and how substrate stiffness affects amount and rate of strain. This new knowledge of cell-substrate interaction can be applied further to research regarding chromatin spatiotemporal dynamics to better understand gene accessibility for transcription. Studying how the cell functions on a deeper level will provide understanding of cellular morphological changes and proliferation. This study uses the methods of optical microscopy and traction force microscopy (TFM) to image substrate deformation as well as analyze its strain profile to find where forces are interacting …