Biomedical Engineering and Bioengineering Commons^™

Histological Characterization Of Inter Ictal Epileptiform Discharges Generating Brain Regions Using A Preclinical Model Of Focal Cortical Dysplasia, Abhay S. Deshmukh

FIU Electronic Theses and Dissertations

Current clinical practice of resective surgery in focal epilepsy involves electroencephalogram (EEG) brain source imaging to localize irritative brain areas from where Inter-ictal epileptiform discharges (IEDs) emerge, useful to localize the seizures-onset zones. Unfortunately, there are no previous systematic studies to characterize the pathophysiological mechanisms and abnormal cellular substrates in these irritative areas since histological data are available only from the final resective zones. To address this issue we applied a combination of EEG brain imaging described by Bae et al. (2015) using cutting-edge technology for high-density scalp EEG in rodents and histological analysis on a chronic rat model of …

Intraoperative Guidance For Pediatric Brain Surgery Based On Optical Techniques, Yinchen Song

FIU Electronic Theses and Dissertations

For most of the patients with brain tumors and/or epilepsy, surgical resection of brain lesions, when applicable, remains one of the optimal treatment options. The success of the surgery hinges on accurate demarcation of neoplastic and epileptogenic brain tissue. The primary goal of this PhD dissertation is to demonstrate the feasibility of using various optical techniques in conjunction with sophisticated signal processing algorithms to differentiate brain tumor and epileptogenic cortex from normal brain tissue intraoperatively.

In this dissertation, a new tissue differentiation algorithm was developed to detect brain tumors in vivo using a probe-based diffuse reflectance spectroscopy system. The system …

Magnetic Resonance Imaging And Histology Correlation In The Neocortex In Temporal Lobe Epilepsy., Maged Goubran, Robert R Hammond, Sandrine De Ribaupierre, Jorge G Burneo, Seyed Mirsattari, David A Steven, Andrew G Parrent, Terry M Peters, Ali R Khan

Robarts Imaging Publications

OBJECTIVE: To investigate the histopathological correlates of quantitative relaxometry and diffusion tensor imaging (DTI) and to determine their efficacy in epileptogenic lesion detection for preoperative evaluation of focal epilepsy.

METHODS: We correlated quantitative relaxometry and DTI with histological features of neuronal density and morphology in 55 regions of the temporal lobe neocortex, selected from 13 patients who underwent epilepsy surgery. We made use of a validated nonrigid image registration protocol to obtain accurate correspondences between in vivo magnetic resonance imaging and histology images.

RESULTS: We found T1 to be a predictor of neuronal density in the neocortical gray matter (GM) …

Registration Of In-Vivo To Ex-Vivo Mri Of Surgically Resected Specimens: A Pipeline For Histology To In-Vivo Registration., Maged Goubran, Sandrine De Ribaupierre, Robert R Hammond, Catherine Currie, Jorge G Burneo, Andrew G Parrent, Terry M Peters, Ali R Khan

Robarts Imaging Publications

BACKGROUND: Advances in MRI have the potential to improve surgical treatment of epilepsy through improved identification and delineation of lesions. However, validation is currently needed to investigate histopathological correlates of these new imaging techniques. The purpose of this work is to develop and evaluate a protocol for deformable image registration of in-vivo to ex-vivo resected brain specimen MRI. This protocol, in conjunction with our previous work on ex-vivo to histology registration, completes a registration pipeline for histology to in-vivo MRI, enabling voxel-based validation of novel and existing MRI techniques with histopathology.

NEW METHOD: A combination of image-based and landmark-based 3D …