Biomedical Engineering and Bioengineering Commons^™

Microarray Embedding/Sectioning For Parallel Analysis Of 3d Cell Spheroids., Jonathan Gabriel, David Brennan, Jennifer H Elisseeff, Vincent Beachley

Henry M. Rowan College of Engineering Faculty Scholarship

Three-dimensional cell spheroid models can be used to predict the effect of drugs and therapeutics and to model tissue development and regeneration. The utility of these models is enhanced by high throughput 3D spheroid culture technologies allowing researchers to efficiently culture numerous spheroids under varied experimental conditions. Detailed analysis of high throughput spheroid culture is much less efficient and generally limited to narrow outputs, such as metabolic viability. We describe a microarray approach that makes traditional histological embedding/sectioning/staining feasible for large 3D cell spheroid sample sets. Detailed methodology to apply this technology is provided. Analysis of the technique validates the …