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Intensification Of Clostridium Pasteurianum Fermentation Producing N-Butanol From Glycerol Using Microfiltration Cell Recycle, Colin W. Couper

Electronic Thesis and Dissertation Repository

This work demonstrates lab scale intensification of the fermentation of glycerol to 1-butanol using Clostridum pasteurianum, starting with simulation and comparison of different cell recycle arrangements, development of a cell recycle apparatus with an existing bioreactor, and demonstration of fermentation with the final system. Fermentations performed with the completed system showed that the cell recycle system was not significantly inhibitory to fermentation, and achieved a maximum apparent cell dry weight of 3.14g/L and a maximum butanol productivity of 1.16g/Lh.