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Full-Text Articles in Biomedical Engineering and Bioengineering
The Role Of Bone Sialoprotein In Periodontal Tissue Development And Bone Repair, Yohannes Soenjaya
The Role Of Bone Sialoprotein In Periodontal Tissue Development And Bone Repair, Yohannes Soenjaya
Electronic Thesis and Dissertation Repository
Bone development and repair involve complex processes that include interaction between cells and their surrounding matrix. In the body, bone sialoprotein (BSP) expression is up-regulated at the onset of mineralization. BSP is a multifunctional acidic phosphoprotein with collagen-binding, hydroxyapatite nucleating, and integrin recognition (RGD sequence, which is important for cell-attachment and signaling) regions. Mice lacking BSP expression (Bsp-/-), exhibit a bone phenotype with reductions in bone mineral density, bone length, osteoclast activation, and impaired bone healing. This thesis examined the role of BSP in tooth development and also its potential use as a therapeutic reagent for bone …
Toward Complete Continuity In Antibody Biomanufacture: Multi-Column Continuous Chromatography For Protein A Capture And Mixed Mode Hydroxyapatite Polishing, Anthony Grabski, Tom Van Oosbree, Beth Hammer, Alla Zilberman, Robert Mierendoff
Toward Complete Continuity In Antibody Biomanufacture: Multi-Column Continuous Chromatography For Protein A Capture And Mixed Mode Hydroxyapatite Polishing, Anthony Grabski, Tom Van Oosbree, Beth Hammer, Alla Zilberman, Robert Mierendoff
Integrated Continuous Biomanufacturing II
Monoclonal antibodies (mAbs) are a predominant modality for a broad range of clinical indications including oncology and inflammatory diseases. Increasing manufacturing capacity and decreasing cost per purification campaign are critical factors for making antibody therapies more affordable. Cell culture mAb expression levels have steadily increased over the past ten years with titers of five grams per liter frequently achieved. Higher titers reduce production costs and allow processing of kilogram quantities for clinical trials from single use cell culture vessels. Drawbacks of increased titers include higher levels of aggregates, fragments, variants, and process impurities. These combined titer and impurity burdens further …
Effect Of Ion Concentration On Mechanosynthesis Of Carbonated Chlorapatite Nanopowders
Effect Of Ion Concentration On Mechanosynthesis Of Carbonated Chlorapatite Nanopowders
Faculty of Engineering University of Malaya
Carbonated chlorapatite nanopowders (n-CCAp) with different degrees of substitution were successfully synthesized by the one-step mechanochemical process. Results demonstrated that the formation of n-CCAp was influenced strongly by the carbonate content (x). From X-ray analysis, crystallite size, crystallinity degree, and unit cell volume of n-CCAp decreased significantly as carbonate content (x) increased from 0 to 2. Conversely, the lattice strain and the volume fraction of grain boundaries grew considerably. Microscopic analysis showed the average particle size of the synthesized powders was 15 +/- 10 nm. The influence of carbonate concentration on mechanosynthesis of pure n-CCAp utilizing a facile solid-state process …
Effect Of High-Energy Ball Milling On The Formation And Micro Structural Features Of Carbonated Chlorapatite Nanopowders
Faculty of Engineering University of Malaya
Carbonated chlorapatite nanopowders (n-CCAp) were synthesized by mechanochemical process from calcite (CaCO3), phosphorus pentoxide (P2O5), and calcium chloride (CaCl2) as raw materials. Results demonstrated that the formation of n-CCAp was influenced strongly by the milling time. At the beginning of milling (up to 15 min), CaCO3 and CaCl2 were the dominant phases, while P2O5 disappeared entirely due to its very high deliquescent nature. With increasing the milling time to 600 min, the progressive mechanochemical reaction was completed which resulted in the formation of nanostructured carbonated chlorapatite. According to the X-ray diffraction data, crystallite size of the product decreased from 24 …