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Biomedical Engineering and Bioengineering Commons™
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- Articles (2)
- 3D culture (1)
- Airway smooth muscle (1)
- Airway wall remodeling (1)
- Amperometry (1)
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- Asthma (1)
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- Electromagnetic fields (1)
- Fibroblast growth factor (FGF2) (1)
- Grip Force (1)
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- Human fibroblasts (1)
- Instrumented Handle (1)
- Magnetophoresis (1)
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- Microfluidic devices (1)
- On-chip recording (1)
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- Thin-film Sensors (1)
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- Vibration (1)
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Articles 1 - 7 of 7
Full-Text Articles in Biomedical Engineering and Bioengineering
Fgf2-Induced Effects On Transcriptome Associated With Regeneration Competence In Adult Human Fibroblasts, Sakthikumar Ambady, Olga Kashpur, David Lapointe, Elizabeth Ryder, Tanja Dominko
Fgf2-Induced Effects On Transcriptome Associated With Regeneration Competence In Adult Human Fibroblasts, Sakthikumar Ambady, Olga Kashpur, David Lapointe, Elizabeth Ryder, Tanja Dominko
Sakthikumar Ambady
Adult human fibroblasts grown in low oxygen and with FGF2 supplementation have the capacity to tip the healing outcome of skeletal muscle injury - by favoring regeneration response in vivo over scar formation. Here, we compare the transcriptomes of control adult human dermal fibroblasts and induced regeneration-competent (iRC) fibroblasts to identify transcriptional changes that may be related to their regeneration competence. RESULTS: We identified a unique gene-expression profile that characterizes FGF2-induced iRC fibroblast phenotype. Significantly differentially expressed genes due to FGF2 treatment were identified and analyzed to determine overrepresented Gene Ontology terms. Genes belonging to extracellular matrix components, adhesion molecules, …
Developing A Small-Footprint Bioengineering Program, Alisha Sarang-Sieminski, Debbie Chachra
Developing A Small-Footprint Bioengineering Program, Alisha Sarang-Sieminski, Debbie Chachra
Alisha L. Sarang-Sieminski
The field of bioengineering is rapidly changing and expanding to include not only more traditional bioengineering applications (e.g. device-focused areas such as prosthetics, imaging) but also more recent sub-fields and technologies(e.g. more biologically-focused areas such as those enabled by tissue engineering and microfluidics). This rapid change, coupled with the intrinsically interdisciplinary nature of bioengineering, presents a unique challenge to the developers of academic programs, as they need to both select relevant content and strike a balance between depth and breadth. We, the architects of the bioengineering program at the undergraduate-only Franklin W. Olin College of Engineering, which enrolled its first …
Computational Design Optimization For Microfluidic Magnetophoresis, Brian Plouffe, Laura Lewis, Shashi Murthy
Computational Design Optimization For Microfluidic Magnetophoresis, Brian Plouffe, Laura Lewis, Shashi Murthy
Shashi K. Murthy
Current macro- and microfluidic approaches for the isolation of mammalian cells are limited in both efficiency and purity. In order to design a robust platform for the enumeration of a target cell population, high collection efficiencies are required. Additionally, the ability to isolate pure populations with minimal biological perturbation and efficient off-chip recovery will enable subcellular analyses of these cells for applications in personalized medicine. Here, a rational design approach for a simple and efficient device that isolates target cell populations via magnetic tagging is presented. In this work, two magnetophoretic microfluidic device designs are described, with optimized dimensions and …
Nonlinear Strain Stiffening Is Not Sufficient To Explain How Far Cells Can Feel On Fibrous Protein Gels., Kristen Billiar, Mathilda Rudnicki, Heather Cirka, Maziar Aghvami, Edward Sander, Qi Wen
Nonlinear Strain Stiffening Is Not Sufficient To Explain How Far Cells Can Feel On Fibrous Protein Gels., Kristen Billiar, Mathilda Rudnicki, Heather Cirka, Maziar Aghvami, Edward Sander, Qi Wen
Kristen L. Billiar
Recent observations suggest that cells on fibrous extracellular matrix materials sense mechanical signals over much larger distances than they do on linearly elastic synthetic materials. In this work, we systematically investigate the distance fibroblasts can sense a rigid boundary through fibrous gels by quantifying the spread areas of human lung fibroblasts and 3T3 fibroblasts cultured on sloped collagen and fibrin gels. The cell areas gradually decrease as gel thickness increases from 0 to 150 μm, with characteristic sensing distances of >65 μm below fibrin and collagen gels, and spreading affected on gels as thick as 150 μm. These results demonstrate …
Parallel Recording Of Neurotransmitters Release From Chromaffin Cells Using A 10 X 10 Cmos Ic Potentiostat Array With On-Chip Working Electrodes, Brian Kim, Adam Herbst, Sung Kim, Bradley Minch, Manfred Lindau
Parallel Recording Of Neurotransmitters Release From Chromaffin Cells Using A 10 X 10 Cmos Ic Potentiostat Array With On-Chip Working Electrodes, Brian Kim, Adam Herbst, Sung Kim, Bradley Minch, Manfred Lindau
Bradley Minch
Neurotransmitter release is modulated by many drugs and molecular manipulations. We present an active CMOS-based electrochemical biosensor array with high throughput capability (100 electrodes) for on-chip amperometric measurement of neurotransmitter release. The high-throughput of the biosensor array will accelerate the data collection needed to determine statistical significance of changes produced under varying conditions, from several weeks to a few hours. The biosensor is designed and fabricated using a combination of CMOS integrated circuit (IC) technology and a photolithography process to incorporate platinum working electrodes on-chip. We demonstrate the operation of an electrode array with integrated high-gain potentiostats and output time-division …
Development And Application Of A Portable System To Reliably Measure Grip Forces Using Thin-Film Force Sensors, Shane Tornifoglio
Development And Application Of A Portable System To Reliably Measure Grip Forces Using Thin-Film Force Sensors, Shane Tornifoglio
Shane Vincent Tornifoglio
Accurate tracking of hand grip force is an important consideration needed for a robust understanding in the study of human biomechanics. One aspect where it proves useful is in hand-arm vibration, such as from gripping a power tool. Depending on how firmly the user is gripping the tool, they may change their exposure levels to the tool vibration, which can lead to potential disorders such as Hand-Arm Vibration Syndrome (HAVS). A small, battery powered portable force unit has been developed to supply power to, and condition, the signals from eight thin-film force sensors. This gives a better understanding of the …
Development And Characterization Of A 3d Multicell Microtissue Culture Model Of Airway Smooth Muscle., Glenn Gaudette, Adrian West, Nishat Zaman, Darren Cole, Matthew Walker, Wesley Legant, Thomas Boudou, Christopher Chen, John Favreau, Elizabeth Cowley, Geoffrey Maksym
Development And Characterization Of A 3d Multicell Microtissue Culture Model Of Airway Smooth Muscle., Glenn Gaudette, Adrian West, Nishat Zaman, Darren Cole, Matthew Walker, Wesley Legant, Thomas Boudou, Christopher Chen, John Favreau, Elizabeth Cowley, Geoffrey Maksym
Glenn R. Gaudette
Airway smooth muscle (ASM) cellular and molecular biology is typically studied with single-cell cultures grown on flat 2D substrates. However, cells in vivo exist as part of complex 3D structures, and it is well established in other cell types that altering substrate geometry exerts potent effects on phenotype and function. These factors may be especially relevant to asthma, a disease characterized by structural remodeling of the airway wall, and highlights a need for more physiologically relevant models of ASM function. We utilized a tissue engineering platform known as microfabricated tissue gauges to develop a 3D culture model of ASM featuring …