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Biomedical Engineering and Bioengineering Commons™
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Full-Text Articles in Biomedical Engineering and Bioengineering
Image Analysis Of Primary Bone-Derived Cells On Different Polystyrene Surfaces, R. M. Shelton, G. Landini
Image Analysis Of Primary Bone-Derived Cells On Different Polystyrene Surfaces, R. M. Shelton, G. Landini
Cells and Materials
The aim of the present study was to examine whether two different cell populations could be discerned using image analysis of a variety of morphological parameters on bacteriological and tissue culture polystyrene surfaces. Rat periosteal and osteoblast cultures were established on both polystyrene petri dishes and examined using phase contrast microscopy after one and two weeks before capturing digital images which were stored on a personal computer. The digital images were processed to identify the cell margins or perimeter, from which seven different morphological parameters were calculated using a program developed (by GL) for both the cell populations on the …
A Quantitative Method Of Measuring Cell-Substrate Adhesion Areas, R. G. Richards, G. Rh Owen, B. A. Rahn, I. Ap Gwynn
A Quantitative Method Of Measuring Cell-Substrate Adhesion Areas, R. G. Richards, G. Rh Owen, B. A. Rahn, I. Ap Gwynn
Cells and Materials
Variability in measurements of the 'cell adhesion strength' of fibroblasts to substrates using mechanical disruption techniques causes difficulty in determining precisely the position, in the cytoskeleton-focal adhesion-matrix -substrate interface, where failure has occurred. In the present study, a quantitative in vitro procedure for measuring the total area and percentage of fibroblast adhesion to biomaterials, using the scanning electron microscope (SEM), is described. The amount of adhesion ofL929 and Balb/c3T3 fibroblasts to discs of stainless steel, commercially pure titanium, and polyethylene terepthalate (Thermanox) was quantified. Cells were fixed, stained with heavy metals, dehydrated and embedded in resin. The resin blocks were …