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Biomedical Engineering and Bioengineering Commons™
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Full-Text Articles in Biomedical Engineering and Bioengineering
Image Analysis Of Primary Bone-Derived Cells On Different Polystyrene Surfaces, R. M. Shelton, G. Landini
Image Analysis Of Primary Bone-Derived Cells On Different Polystyrene Surfaces, R. M. Shelton, G. Landini
Cells and Materials
The aim of the present study was to examine whether two different cell populations could be discerned using image analysis of a variety of morphological parameters on bacteriological and tissue culture polystyrene surfaces. Rat periosteal and osteoblast cultures were established on both polystyrene petri dishes and examined using phase contrast microscopy after one and two weeks before capturing digital images which were stored on a personal computer. The digital images were processed to identify the cell margins or perimeter, from which seven different morphological parameters were calculated using a program developed (by GL) for both the cell populations on the …
A Novel In Vitro Model To Study The Calcification Of Biomaterials, Q. Liu, J. Weng, J. G. C. Wolke, J. R. De Wijn, C. A. Van Blitterswijk
A Novel In Vitro Model To Study The Calcification Of Biomaterials, Q. Liu, J. Weng, J. G. C. Wolke, J. R. De Wijn, C. A. Van Blitterswijk
Cells and Materials
A novel in vitro model based on a solution mainly composed of sodium, calcium, chloride and phosphate ions, was developed to study the calcification of biomaterials at near physiological conditions. This model, due to its ability to quickly calcify the tested materials, is called Accelerated Calcification Solution (ACS). Polyactive ™ 30170, Polyactive™ 70/30 and its composites with nano-apatite were used as testing materials because of their known calcification behaviour. The results showed that Polyactive™ 70/30 and its composites could calcify in ACS in a relatively short period, while the polymer without filler failed to induce calcium phosphate precipitation in more …