Biomedical Engineering and Bioengineering Commons^™

Beta-Tricalcium Phosphate Resorption By Monocytes In Biphasic Calcium Phosphate: An In Vitro Study, Wolf Bohne, Jean A. Pouëzat, Dorothea Pouëzat, Guy Daculsi

Cells and Materials

A macroporous biphasic calcium phosphate (BCP) of 60.7% carbonated dense non-porous hydroxyapatite (HA)/39.3% dense microporous ß-tricalcium phosphate (ß-TCP) was immersed during 15 days in a supplemented a-MEM (minimal essential medium) solution with and without dog bone marrow cells obtained by punction. The aim of the study was to demonstrate the time-related changes in the BCP fractions by means of X -ray diffraction, infrared spectroscopy and scanning electron microscopy, and to investigate the cell populations. The HA/ß-TCP ratios varied according to immersion duration and cell presence. If any, there was a slight preponderance of ß-TCP …