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Biomedical Engineering and Bioengineering Commons™
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Full-Text Articles in Biomedical Engineering and Bioengineering
Applications Of Fourier Transform Infrared (Ft-Ir) Microscopy To The Study Of Mineralization In Bone And Cartilage, Adele L. Boskey, Nancy Pleshko, Stephen B. Doty, Richard Mendelsohn
Applications Of Fourier Transform Infrared (Ft-Ir) Microscopy To The Study Of Mineralization In Bone And Cartilage, Adele L. Boskey, Nancy Pleshko, Stephen B. Doty, Richard Mendelsohn
Cells and Materials
Knowledge of the phase, composition, and crystallite size and perfection of the mineral in normal and abnormally calcified tissues provides insight into the mechanism by which this mineral was deposited. These data also can be used to develop rational therapies for pathological conditions characterized by abnormal mineral deposition. As illustrated in this review, coupling of an optical microscope with a Fourier transform infrared (FT-IR) spectrophotometer permits the mapping at 20 μm spatial resolution of changes in mineral characteristics (content, particle size, composition) in the growth plate, in bone biopsies, in mineralizing cell culture systems, and in soft tissue calcifications. Based …
Comparative Assessment Of The Cytotoxicity Of Various Substrates In Organ Culture And Cell Culture: A Scanning Electron Microscopy Study, J. L. Duval, R. Warocquier-Clerout, M. F. Sigot-Luizard
Comparative Assessment Of The Cytotoxicity Of Various Substrates In Organ Culture And Cell Culture: A Scanning Electron Microscopy Study, J. L. Duval, R. Warocquier-Clerout, M. F. Sigot-Luizard
Cells and Materials
A comparative study of the behavior of chick embryo endothelial cells grown on various substrates was performed in order to establish the reliability and the limitation of both cell and organ culture methods. Following substrates were analyzed to compare these two different culture techniques: bovine serum albumin, pigskin gelatin and albumin + gelatin cross-linked by glutaraldehyde or carbodiimide, fibrin glue and negative control (Thermanox*). Parameters of cell growth and adhesion were calculated and compared with electron microscopic observations of cell morphology and of the extracellular matrix. Both culture methods provided complementary results and led to a similar classification of the …