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Biomedical Engineering and Bioengineering Commons™
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Articles 1 - 5 of 5
Full-Text Articles in Biomedical Engineering and Bioengineering
Changes In Expired End-Tidal Carbon Dioxide During Cardiopulmonary Resuscitation In Dogs: A Prognostic Guide For Resuscitation Efforts, Karl B. Kern, Arthur B. Sanders, William D. Voorhees, Charles F. Babbs, Willis A. Tacker, Gordon A. Ewy
Changes In Expired End-Tidal Carbon Dioxide During Cardiopulmonary Resuscitation In Dogs: A Prognostic Guide For Resuscitation Efforts, Karl B. Kern, Arthur B. Sanders, William D. Voorhees, Charles F. Babbs, Willis A. Tacker, Gordon A. Ewy
Weldon School of Biomedical Engineering Faculty Publications
Expired end-tidal carbon dioxide (PCO2) measurements made during cardiopulmonary resuscitation have correlated with cardiac output and coronary perfusion pressure when wide ranges of blood flow are included. The utility of such measurements for predicting resuscitation outcome during the low flow state associated with closed chest cardiopulmonary resuscitation remains uncertain. Expired end-tidal PCO2 and coronary perfusion pressures were measured in 15 mongrel dogs undergoing 15 min of closed chest cardiopulmonary resuscitation after a 3 min period of untreated ventricular fibrillation. In six successfully resuscitated dogs, the mean expired end-tidal PCO2 was significantly higher than that in nine nonresuscitated dogs only after …
Lethal Hydroxyl Radical Production In Paraquat-Treated Plants, Charles F. Babbs, Jo Ann Pham, Ronald C. Coolbaugh
Lethal Hydroxyl Radical Production In Paraquat-Treated Plants, Charles F. Babbs, Jo Ann Pham, Ronald C. Coolbaugh
Weldon School of Biomedical Engineering Faculty Publications
Bipyridinium herbicides, including paraquat and diquat, are believed to act by generating highly reactive, oxygen-centered free radicals within chloroplasts when treated plants are exposed to sunlight. This hypothesis has not yet been confirmed by direct chemical measurements of specific free radicals. We studied paraquat-treated plants using a new method able to detect and quantify formation of highly reactive and deleterious hydroxyl radicals (HO*), in which dimethyl sulfoxide (DMSO) is used as a molecular probe. DMSO is oxidized by HO* to form the stable, nonradical compound, methane sulfinic acid, which can be easily extracted from plant tissue and measured spectrophotometrically. Initial …
Scatchard Analysis Of Methane Sulfinic Acid Production From Dimethyl Sulfoxide: A Method To Quantify Hydroxyl Radical Formation In Physiologic Systems, Charles F. Babbs, David W. Griffin
Scatchard Analysis Of Methane Sulfinic Acid Production From Dimethyl Sulfoxide: A Method To Quantify Hydroxyl Radical Formation In Physiologic Systems, Charles F. Babbs, David W. Griffin
Weldon School of Biomedical Engineering Faculty Publications
A major impediment to the confirmation of free radical mechanisms in pathogenesis is a lack of direct, chemical evidence that oxygen centered free radicals actually arise in living tissues in quantities sufficient to cause serious damage. This investigation was conducted to validate the use of dimethyl sulfoxide (DMSO) as a quantitative molecular probe for the generation of hydroxyl radicals (HO*) under physiologic conditions. Reaction of HO* with DMSO produces methane sulfinic acid (MSA) as a primary product, which can be detected by a simple colorimetric assay. To develop a method for estimating total HO* production, we studied two model systems: …
Effect Of Oxygen Concentration On The Formation Of Malondialdehyde-Like Material In A Model Of Tissue Ischemia And Reoxygenation, Steven C. Salaris, Charles F. Babbs
Effect Of Oxygen Concentration On The Formation Of Malondialdehyde-Like Material In A Model Of Tissue Ischemia And Reoxygenation, Steven C. Salaris, Charles F. Babbs
Weldon School of Biomedical Engineering Faculty Publications
This study was conducted to explore the functional relationship between oxygen concentration during tissue reoxygenation after ischemia and the extent of postischemic lipid peroxidation, an indicator of reoxygenation injury. Excised rat liver or kidney tissue was rendered ischemic for 1 h at 37°C, minced into 1 mm3 fragments, and then reoxygenated for 1 h in flasks of buffered salt solution containing various amounts of oxygen. Production of malondialdehyde-like material (MDA) was measured to indicate lipid peroxidation. MDA production was minimal at oxygen tensions less than 10 mmHg, increased sharply from 10 to 50 mmHg, and plateaued at approximately 100 mmHg. …
Quantitative Effects Of Iron Chelators On Hydroxyl Radical Production By The Superoxide-Driven Fenton Raction, Jean Blair Smith, Joanne C. Cusumano, Charles F. Babbs
Quantitative Effects Of Iron Chelators On Hydroxyl Radical Production By The Superoxide-Driven Fenton Raction, Jean Blair Smith, Joanne C. Cusumano, Charles F. Babbs
Weldon School of Biomedical Engineering Faculty Publications
Iron bound to certain chelators is known to promote the conversion of superoxide radicals (O2) to hydroxyl radicals (HO') by the superoxide-driven Fenton reaction. The production of HO' by various iron chelates was studied using the reaction of dimethyl sulfoxide and HO' to produce methane sulphinic acid. Methane sulphinic acid was quantified by use of a simple colorimetric assay and used to determine the amounts of HO' produced. Superoxide was generated from 200 M hypoxanthine and 0.05 U/ml xanthine oxidase in the presence of 0-100 M iron and 100 M of each chelator. The results of this preliminary investigation illustrate …