Biomedical Engineering and Bioengineering Commons^™

A Pulsatile Bioreactor For Conditioning Tissue Engineered Heart Valves, Leslie Sierad

All Theses

Tissue engineered constructs with autologous adult stem cells capable of self-repair and growth are highly desired replacements for diseased heart valves. However, the current approaches have inadequate mechanical properties to withstand in vivo implantation. Therefore, our group hypothesized that an in vitro environment of physiological intra-cardiac pressures and flow will stimulate stem cells to differentiate and remodel valvular scaffold constructs before implantation.
The group developed a pneumatic-driven conditioning system (Aim I) consisting of a three-chambered heart valve bioreactor, a pressurized compliance tank, a reservoir tank, one-way valves, pressure-retaining valves, and pressure transducers. The system can be sterilized using conventional autoclaving …