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Full-Text Articles in Biomedical Engineering and Bioengineering
Fabrication And Characterization Of An Extracellular Matrix Hydrogel For Aortic Valve Applications, Brady Culbreth
Fabrication And Characterization Of An Extracellular Matrix Hydrogel For Aortic Valve Applications, Brady Culbreth
All Theses
With an estimated 5 million people suffering from valve disease in the United, valve disease is currently the leading cause of cardiovascular disease. Each year, between 80,000 and 85,000 aortic valve replacements are performed in order to treat the stenotic heart valves. Despite this being a worldwide epidemic, the current valve replacement options that are on the market have distinct limitations. Furthermore, a viable alternative does not exist for the patients that are not candidates for the current treatment methods. Our proposed solution to this epidemic is to create a highly viable injectable scaffold that would allow for the minimally …
A Pulsatile Bioreactor For Conditioning Tissue Engineered Heart Valves, Leslie Sierad
A Pulsatile Bioreactor For Conditioning Tissue Engineered Heart Valves, Leslie Sierad
All Theses
Tissue engineered constructs with autologous adult stem cells capable of self-repair and growth are highly desired replacements for diseased heart valves. However, the current approaches have inadequate mechanical properties to withstand in vivo implantation. Therefore, our group hypothesized that an in vitro environment of physiological intra-cardiac pressures and flow will stimulate stem cells to differentiate and remodel valvular scaffold constructs before implantation.
The group developed a pneumatic-driven conditioning system (Aim I) consisting of a three-chambered heart valve bioreactor, a pressurized compliance tank, a reservoir tank, one-way valves, pressure-retaining valves, and pressure transducers. The system can be sterilized using conventional autoclaving …
Development Of A Cellular Fiber Spinning Technology For Regenerative Medicine, Willie Jones
Development Of A Cellular Fiber Spinning Technology For Regenerative Medicine, Willie Jones
All Theses
In order to prove that our textile hollow fiber spinning apparatus was adequate for cellular encapsulation, we confirmed the viability and metabolic activity of green fluorescent protein (GFP) labeled bovine mammary epithelial cells (MAC-Ts) that were encapsulated in either the wall or lumen of alginate fibers. After a 21 day in vitro macroscopic evaluation, no decrease in fluorescence was observed; and it was determined that MAC-Ts encapsulated under both methods produced lactic acid and consumed glucose. Histomorphological analyses revealed that the diameter of the MAC-Ts increased under both encapsulation scenarios, with little to no evidence of cell cluster propagation. Based …