Biomedical Engineering and Bioengineering Commons^™

Surface-Functionalized Silica Nanocarriers For Mitigating Water Stress In Wheat And Benefiting The Root Microbiome, Anthony Cartwright

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Changes in climate and shifting patterns of drought threaten the growth of important cash crops like wheat. The element silicon serves as a plant nutrient and shows promise for strengthening wheat against drought while remaining safe to both the crop and the positive bacteria that grow on its roots. Silicon can be added to wheat in the form of silicon-dioxide nanoparticles featuring protective coatings made from plant-beneficial nutrients. These nanoparticles can be engineered with high surface area or porous structures allowing them to be loaded with additional nutrients that can be delivered to crops. In a laboratory setting, such nanoparticles …

Cell Imaging And Data Analysis For Biomaterial-Mammalian Co-Cultures, Jefferson Pontsler

All Graduate Plan B and other Reports, Spring 1920 to Spring 2023

Microscopic bioimaging is a useful approach to study cell-biomaterial interactions which are vital to the biomedical application of biomaterials. Through microscopic imaging, numerous cellular responses, such as proliferation, uptake, and death, can be recorded, characterized and analyzed.

In this thesis, I first provided basic introductions to the imaging techniques and analysis tools, especially those that are highly relevant to the studies of biomaterials and cell interactions. I also detailed the adaptation of these techniques and tools in the application of two specific research projects in biomaterials, with special focuses on the imaging and analysis.

The first project assessed the subtle …

Silane Modulation Of Protein Conformation And Self-Assembly, Abul Bashar Mohammad Giasuddin

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This research focused on development of nanoparticle- based therapeutics against amyloid fibrils. Amyloid fibrils are associated with various diseases such as Parkinson’s, Huntington’s, mad cow disease, Alzheimer’s, and cataracts. Amyloid fibrils develop when proteins change their shape from a native form to a pathogenic “misfolded” form. The misfolded proteins have the ability to recruit more native proteins into the pathogenic forms, which self-assemble into amyloid fibrils that are hallmarks of the various protein-misfolding diseases listed above. Amyloid fibrils are highly resistant to degradation, which may contribute to the symptoms of amyloid diseases. Synthetic drugs, natural compounds, and antibodies are widely …