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Biomedical Engineering and Bioengineering Commons™
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Full-Text Articles in Biomedical Engineering and Bioengineering
Modeling, Design, And Validation Of Fluorescent Spherical Enzymatic Glucose Microsensors Using Nanoengineered Polyelectrolyte Coatings, Jonathan Quincy Brown
Modeling, Design, And Validation Of Fluorescent Spherical Enzymatic Glucose Microsensors Using Nanoengineered Polyelectrolyte Coatings, Jonathan Quincy Brown
Doctoral Dissertations
In this dissertation, the modeling, design, and function of fluorescent spherical enzymatic microsensors for minimally-invasive diabetic monitoring are described. The devices reported herein are novel and their experimental construction and theoretical analysis have not been previously reported, thus laying the foundation for an intensive set of studies. These sensors are based on the encapsulation of an enzymatic fluorescent assay for glucose within hydrogel alginate microspheres with diameters on the order of tens of microns, which are of the appropriate size for intradermal implantation. A novel feature of these sensors is the use of multifunctional nanoengineered ultrathin multilayer polyelectrolyte coatings on …
Application Of Self -Assembled Ultrathin Film Coatings To Stabilize Enzyme Encapsulation And Activity In Alginate Microspheres, Rohit Srivastava
Application Of Self -Assembled Ultrathin Film Coatings To Stabilize Enzyme Encapsulation And Activity In Alginate Microspheres, Rohit Srivastava
Doctoral Dissertations
Alginate-based hydrogels have been used for the encapsulation of a variety of materials, including enzymes, proteins, and cells for a wide range of applications from drug delivery to biosensors and bioreactors. However, due to the high porosity of the matrix, it has been increasingly difficult to retain macromolecules inside the alginate matrix, leading to loss in functionality over time. In an effort to improve the stability for long-term biosensor use, this work investigated layer-by-layer self-assembly as a potential technique to provide a diffusion barrier to an encapsulated macromolecule. Alginate microspheres (∼2–50μm radius) were fabricated using an emulsification technique, and were …