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Biomedical Engineering and Bioengineering Commons™
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Full-Text Articles in Biomedical Engineering and Bioengineering
Gold Nanoparticle Enhancements In Electroporation Mediated Dna And Rna Therapeutics, Shuyan Huang
Gold Nanoparticle Enhancements In Electroporation Mediated Dna And Rna Therapeutics, Shuyan Huang
Doctoral Dissertations
Nonviral gene delivery methods have been explored as the replacement of viral systems for their low toxicity and immunogenicity. However, they have yet to reach levels competitive to their viral counterparts. Electroporation figured prominently as an effective nonviral gene delivery approach for its balance on the transfection efficiency and cell viability, no restrictions of probe or cell type, and operation simplicity. The commercial electroporation systems have been widely adopted in the past two decades but still carry drawbacks associated with the high applied electric voltage, unsatisfied delivery efficiency, and/or low cell viability. What we did was to improve electroporation performance …
Impedance Measurements As A Means To Improve The Biological Response Of Gene Electrotransfer, Lina Fajardo Gomez
Impedance Measurements As A Means To Improve The Biological Response Of Gene Electrotransfer, Lina Fajardo Gomez
USF Tampa Graduate Theses and Dissertations
When an electric field is locally applied to tissues in vivo the uptake of exogenous DNA can be greatly increased. This approach to gene transfer, called electroporation (EP) or gene electro transfer (GET), has potential applications in the treatment of skin disorders, vaccinations, some types of cancer and metabolic diseases. The eect of electric elds on cells and tissues has been studied and related to the uptake of DNA. Tissue impedance changes have been measured as a result of electroporation. The aim of this study is to explore the predictive accuracy of impedance spectroscopy for the success of GET. Mice …