Biomedical Engineering and Bioengineering Commons^™

Bacterial Cellulose Templates For Nano-Hydroxyapatite Fibre Synthesis, Jordan A. Demello

Electronic Thesis and Dissertation Repository

Guided bone regeneration is a medical procedure which induces in vivo re-growth of bone using membranes and osteopromoting fillers. In this work, bacterial cellulose fibers were isolated and used as a basis for biomimetic hydroxyapatite growth, with the ultimate goal of producing GBR filler materials. Acetobacter xylinum generated BC using various carbon sources. Fibers were treated with phosphoric acid to phosphorylate functional groups and preconditioned with calcium to nucleate the HA. Simulated body fluid (SBF) furthered the growth. Over 14 days, the product was characterized via EDX, SEM, FTIR, and XRD. The effect of media composition, phosphorylation time, pretreatment, and …