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Biomedical Engineering and Bioengineering Commons™
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Full-Text Articles in Biomedical Engineering and Bioengineering
Controlled Codelivery Of Mir-26a And Antagomir-133a With Osteoconductive Scaffolds To Promote Healing Of Large Bone Defects, Cole J. Ferreira
Controlled Codelivery Of Mir-26a And Antagomir-133a With Osteoconductive Scaffolds To Promote Healing Of Large Bone Defects, Cole J. Ferreira
Masters Theses
Often caused by trauma or tumor removal, large bone defects frequently result in delayed or non-union. The current gold standard for treatment is autograft. However, due to limitations, such as the size and location of the defect, these cannot always be utilized. A common alternative to autograft is the use of BMP-2 with a collagen scaffold, however, this treatment is limited by numerous side effects. In recent years, genetic materials such as microRNAs (miRNAs) have offered possible alternative therapies. MiRNAs are small non-coding RNA molecules that generally range from 20-24 nucleotides, serve as repressors of gene expression, and are involved …
Promoting Extracellular Matrix Crosslinking In Synthetic Hydrogels, Marcos M. Manganare
Promoting Extracellular Matrix Crosslinking In Synthetic Hydrogels, Marcos M. Manganare
Masters Theses
The extracellular matrix (ECM) provides mechanical and biochemical support to tissues and cells. It is crucial for cell attachment, differentiation, and migration, as well as for ailment-associated processes such as angiogenesis, metastases and cancer development. An approach to study these phenomena is through emulation of the ECM by synthetic gels constructed of natural polymers, such as collagen and fibronectin, or simple but tunable materials such as poly(ethylene glycol) (PEG) crosslinked with short peptide sequences susceptible to digestion by metalloproteases and cell-binding domains. Our lab uses PEG gels to study cell behavior in three dimensions (3D). Although this system fosters cell …
Metabolic Modeling Of Secondary Metabolism In Plant Systems, Lisa M. Leone
Metabolic Modeling Of Secondary Metabolism In Plant Systems, Lisa M. Leone
Masters Theses
In the first part of this research, we constructed a Genome scale Metabolic Model (GEM) of Taxus cuspidata, a medicinal plant used to produce paclitaxel (Taxol®). The construction of the T. cuspidata GEM was predicated on recent acquisition of a transcriptome of T. cuspidata metabolism under methyl jasmonate (MJ) elicited conditions (when paclitaxel is produced) and unelicited conditions (when paclitaxel is not produced). Construction of the draft model, in which transcriptomic data from elicited and unelicited conditions were included, utilized tools including the ModelSEED developed by Argonne National Laboratory. Although a model was successfully created and gapfilled by ModelSEED …