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Full-Text Articles in Biomedical Engineering and Bioengineering
Design Of A Modular Endothelialized Platform For Vascularized Bone Regeneration, Kevin Barrett Miles
Design Of A Modular Endothelialized Platform For Vascularized Bone Regeneration, Kevin Barrett Miles
Wayne State University Dissertations
The gold standard for bone regeneration requires harvesting a piece of healthy bone from the patient through a painful procedure, but this piece of autologous bone graft contains the bone and endothelial cells required to rapidly regenerate bone in a defect. We have developed a modular bone regeneration platform, composed of mesenchymal stem cells (MSCs) and hydroxyapatite (HAP) microgranules encapsulated in microcapsules, to replace autologous graft harvesting. The microcapsules are composed of a polyelectrolyte membrane formed by the ionic-complex reaction between chondroitin 4-sulfate (C4S) and chitosan. The specific aims of this thesis were to 1) examine the ability of C4S/HAP/chitosan …
Development Of Scaffold Architectures And Heterotypic Cell Systems For Hepatocyte Transplantation, Dalia Alzebdeh
Development Of Scaffold Architectures And Heterotypic Cell Systems For Hepatocyte Transplantation, Dalia Alzebdeh
Wayne State University Dissertations
In vitro assembly of functional liver tissue is needed to enable the transplantation of tissue-engineered livers. In addition, there is an increasing demand for in vitro models that replicate complex events occurring in the liver. However, tissue engineering of sizable implantable liver systems is currently limited by the difficulty of assembling three dimensional hepatocyte cultures of a useful size, while maintaining full cell viability, an issue which is closely related to the high metabolic rate of hepatocytes. In this study, we first compared two designs of highly porous chitosan-heparin scaffolds seeded with hepatocytes in dynamic perfusion bioreactor systems. The aim …