Biomedical Engineering and Bioengineering Commons^™

Engineering Of Polyketide Biosynthetic Pathways For Bioactive Molecules, Siyuan Wang

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study is focused on engineering of natural product biosynthetic pathways for efficient production of pharmaceutically important molecules or generation of new bioactive molecules for drug development.

Plant natural products are an important source of therapeutics, such as paclitaxel (anticancer) and artemisinin (anti-malarial). Production of plant natural products relies on conventional plant cultivation and solvent extraction, which is time-consuming and cost-ineffective. This work built a biosynthetic platform in Escherichia coli using seven biosynthetic genes from plants and bacteria, which were used to make valuable compounds such as the strong antioxidant resveratrol and anti-inflammatory agent curcuminoids. Through different combinations of these …