Biomedical Engineering and Bioengineering Commons^™

Characterization Of Rhodopseudomonas Palustrus Strains For The Production Of Fixed Nitrogen Fertilizer For Mars, Daniel Kade Derrick

Undergraduate Honors Capstone Projects

The purpose of this project was to characterize the potential of various strains of purple non-sulfur bacteria for the production of fixed nitrogen fertilizers for the manned Mars missions. Six strains of Rhodopseudomonas palustris (CGA009, CGA010, TIE-1, NifA*, and PB23) were all investigated. Through initial growth trial experiments, R. palustris NifA* and PB23 were selected for their engineered nitrogen fixation and rapid growth respectively. Growth curves and ammonium concentrations were collected over time in pilot scale batch photobioreactors (200 mL). Biomass production was then scaled up to benchtop photobioreactors (1.5 L) and then 14 L total volume for both NifA* …

Characterization Of Rhodopseudomonas Palustris Strains For The Production Of Fixed Nitrogen Fertilizer For Mars, Andrew James Walters

Undergraduate Honors Capstone Projects

The purpose of this project was to characterize the potential of various strains of purple non-sulfur bacteria for the production of fixed nitrogen fertilizers for the manned Mars missions. Six strains of Rhodopseudomonas palustris (CGA009, CGA010, TIE-1, NifA*, and PB23) were all investigated. Through initial growth trial experiments, R. palustris NifA* and PB23 were selected for their engineered nitrogen fixation and rapid growth respectively. Growth curves and ammonium concentrations were collected over time in pilot scale batch photobioreactors (200 mL). Biomass production was then scaled up to benchtop photobioreactors (1.5 L). Fourteen liters of both NifA* and PB23 were then …

Oxidative Protection Using Pluronic Micelles, Mitchell Glen Heap

Undergraduate Honors Capstone Projects

Methods for drug delivery are currently being researched in many different applications. One significant obstacle to drug delivery is a drug's inherent hydrophobic character. Drugs that are hydrophobic are difficult to successfully administer since the body consists mostly of water. Therefore, a greater variety of drugs could potentially be used for the treatment of disease if they could successfully be administered. Recent research in oncology and virology has attempted to overcome the obstacles posed by hydrophobic drugs by encapsulating them in micelles. Micelles form spontaneously from compounds that contain both hydrophilic and hydrophobic regions and can be used to increase …

Designing A Synthetic Spider Silk-Based Coating For Urinary Catheters To Reduce The Risk Of Cautis, Alexander Cook

Undergraduate Honors Capstone Projects

Urinary and Intravenous (IV) catheters are two of the most commonly used medical devices for administering vital drugs and obtaining diagnostic samples from patients. Unfortunately, hospital acquired infections (HAIs) occur at alarming rates due to catheter usage. The aim of this project was to design a urinary catheter that would decrease the occurrence of these infections with a catheter coating that utilizes the antimicrobial properties of synthetic spider silk in combination with the antiseptic chlorhexidine. This synthetic spider silk catheter will introduce a novel approach to catheterization in the medical industry.

Designing A Synthetic Spider Silk Based Coating For Urinary Catheters To Reduce The Risk Of Cautis, Tess Marie Armbrust

Undergraduate Honors Capstone Projects

Catheter associated urinary tract infections (CAUTis) are a leading cause of morbidity and mortality in patients with life-threatening conditions. Foley catheters are the most common catheter used when a patient requires hospitalization but can become burdened with microbes which lead to CAUTis, the most common source of hospital acquired infection (HAI). The attached bacteria can form biofilms on the catheter which are difficult to treat using oral antibiotics and can lead to chronic infections. Synthetic spider silk as a catheter coating shows great promise because of demonstrated strength, biocompatibility, and antimicrobial properties. In addition, the antiseptic chlorhexidine can enhance the …

Pairing Of Anaerobic And Aerobic Treatment Of Petroleum Wastewater, Zachary Fica

Undergraduate Honors Capstone Projects

The objective of this project was to treat petroleum refinery wastewater using a combination of anaerobic and aerobic processes, namely an Up-flow Anaerobic Sludge Blanket (UASB) reactor paired with a Rotation Algae Biofilm Reactor (RABR), respectively, to produce a treated effluent. The treatment method developed needed to produce a cost-effective and efficient way to decrease nitrogen, phosphorous, total suspended solids (TSS), and COD concentrations to below State of Utah limitations. It was demonstrated that RABR treatment was capable of reducing effluent concentrations of nitrogen, phosphorus, and TSS to State of Utah limitations. RABR treatment did not significantly reduce COD from …

Amending Phb With Algal Biomass To Enhance Biodegradability, Amanda R. Stoudt

Undergraduate Honors Capstone Projects

Pollution due to petroleum-based plastic is a growing problem all over the world. Petroleum-based plastics that fill landfills and oceans take hundreds of years to degrade. One possible solution to this growing problem is to increase the use of bioplastics. Polyhydroxybutyrate (PHB) is a widely studied bioplastic that is biodegradable in both soil and marine environments. However, PHB use is limited due to its poor mechanical properties. Past researchers have investigated the use of natural additives, primarily different types of plant fibers, to enhance both the mechanical properties and degradation rates of bioplastics. The purpose of this project was to …

Electrospun Spiderskin Bandage For Epidermal Protection And Recovery, Michael David Paskett

Undergraduate Honors Capstone Projects

Spider silk is one of nature's most promising biomaterial s for a variety of applications, however, due to the inability to farm spiders, transgenic hosts are required for large-scale production. With the unique combination of strength, elasticity, and biocompatibility, spider silk has an incredible potential for use in the human body. This study was conducted to merge two major applications of spider silk for the creation of a novel bandaging product. Electrospinning technology was utilized to create a spider silk/polymer bandage matrix to be applied with an aqueous spider silk skin adhesive.

In designing the bandaging matrix , the mechanical …

Measuring The Effects Of High-Altitude Flight And Upper Atmospheric Radiation On Muscle Cells, Annelise Dykes

Undergraduate Honors Capstone Projects

There are several physiological barriers to long-term space travel, including the effects of launch, landing, and microgravity on muscle cells. A payload capsule was designed to maintain cell growth during a high altitude balloon flight to model some of these physiological processes. Murine muscle cells (strain C2C12) were cultured and launched in a capsule on a balloon satellite in November 2016. Cells were monitored for changes due to temperature, flight motion, radiation, and gravity differences by quantifying cell characteristics before and after the flight using physical measurements and cell viability. Instruments were selected to monitor flight data, and a payload …

Targeted Drug Delivery System For Kidney And/Or Liver Failure Patients Using Human Serum Albumin, Sean Bedingfield

Undergraduate Honors Capstone Projects

Compromised liver and/or kidney function reduces the acceptable dosage of a variety of medications that can be administered to patients. These patients still have a need for drugs such as nonsteroidal anti-inflammatory drugs (NSAIDs), antivirals, and antibiotics. The project goal is to provide a drug delivery system to accommodate these reduced dosage limits with added therapeutic benefits to address symptoms of liver or kidney failure. Localized drug delivery allows for a smaller, concentrated dose rather than inundating the patient's system with the drug of interest. Human serum albumin (HSA) is a researched candidate for drug delivery with therapeutic properties. HSA …

Designing An Artificial Tendon/Graft Derived From Silkworm Silk And Synthetic Spider Silk With Respect To Structure, Mechanical Properties, Biocompatibility, And Attachment, Neal Hengge

Undergraduate Honors Capstone Projects

The Achilles tendon is one of the most commonly damaged tendons in the human body (Hansen et al., 2012). Current surgical and therapeutic techniques require extended recovery time and the tendon is rarely returned to its original healthy state. For athletes in particular, strenuous rehabilitation is required to return from a serious injury like a ruptured Achilles tendon (Rosso et al., 2013).

Despite the high amount of sources for grafts to aid in surgical repair, all of the options have drawbacks such as availability, immune response, or poor mechanical properties. Spider silk has the potential to enhance current Achilles tendon …

Combating Antibiotic Resistance Through Development Of A Novel Antimicrobial Delivery Vehicle, Alex Hatch

Undergraduate Honors Capstone Projects

A thermoreversible gel for antimicrobial agent delivery would provide a novel platform suitable for medical applications from persistent infections arising from localized biofilms. Thermoreversible gels could be used to coat medical implants, to inject for subdermal treatments, or to implement in topical applications. FDA approved Pluronics® form thermoreversible gels and certain Pluronics® display inherent varying degrees of antimicrobial activity. Pluronics® have been shown to affect efflux pumps, and reduce ATP levels within the bacterial cell. In combination with antibiotics and other pharmaceuticals, Pluronics® have been used to sequester drugs and act as a delivery vehicle. With advances in nanomedicine, nanoparticles …