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Full-Text Articles in Biomedical Engineering and Bioengineering
Advancing Automated Methods For Microrna Profiling, Kia N. Zellars
Advancing Automated Methods For Microrna Profiling, Kia N. Zellars
Theses and Dissertations
The widespread influence of miRNAs in many biological processes and characteristic changes in expression profiles make them potential biomarkers for a range of diseases. With recent discoveries highlighting the enrichment of particular circulating miRNAs in plasma under pathological conditions, there has been a push for technological advances in miRNA profiling studies as a diagnostic tool. A standardized, automated, high-throughput method for miRNA isolation from human plasma samples was developed in this study. Samples were subject to full miRNome profiling utilizing RT-qPCR. These unique findings demonstrate that developing a distinct miRNA biomarker profile for heart failure patients is feasible and holds …
Automated Image Analysis And Spatial Computational Modeling Of Nf-Kb In Cerebrovascular Endothelial Cells, Kasey Catalfomo
Automated Image Analysis And Spatial Computational Modeling Of Nf-Kb In Cerebrovascular Endothelial Cells, Kasey Catalfomo
Theses and Dissertations
Cerebrovascular endothelial cells play a key part in the inflammatory response of the blood-brain barrier in pathological conditions such as Alzheimer’s disease. Specifically, the NF-κB signaling pathway plays a central role. Better understanding of the factors in inflammatory disease progression can lead to more effective treatments for such devastating illnesses like Alzheimer’s, asthma, arthritis, cancer, diabetes and many more inflammatory diseases. The proposed approach analyzes spatial NF-κB distribution contained in multispectral stacked micrograph images of cerebrovascular endothelial cells indexed based on dose of the activating protein and the length of activation. Image analysis code identifies the location of nuclear boundaries …