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Ex-Vivo Slaughterhouse Porcine Crystalloid-Perfused Beating Heart Via Langendorff Method, Rahiemin Talukder

Electronic Theses and Dissertations

The objective of this study was to resuscitate isolated beating porcine hearts obtained from slaughterhouse swine for a minimum of 1 hour with a crystalloid buffer reperfusion instead of blood via an extracorporeal Langendorff apparatus. The isolated beating heart functions outside of the body under simulated physiologic conditions. Live functional anatomy was controlled under benchtop experimental settings. Porcine hearts (26 total) were topically cooled with saline. 300mL of 25°C cardioplegia with 20KU Streptokinase was preflushed via aortic root. WIT was less than 5 minutes. 1L 4°C cardioplegia was flushed antegrade with aortic cannula (Group 1) or coronary catheterization (Group 2). …