Open Access. Powered by Scholars. Published by Universities.®
Biomedical Engineering and Bioengineering Commons™
Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 7 of 7
Full-Text Articles in Biomedical Engineering and Bioengineering
Decellularized Bovine Nucleus Pulposus As A Biomimetic Scaffold For Intervertebral Disc Regeneration, Christopher Fernandez
Decellularized Bovine Nucleus Pulposus As A Biomimetic Scaffold For Intervertebral Disc Regeneration, Christopher Fernandez
All Theses
Low back pain (LBP) is a significant burden with a lifetime prevalence of 84% and estimated expenditures of $85.9 billion.[1,2] Although LBP is multifactorial; it has been shown that LBP can originate from degenerating intervertebral discs (IVDs); a process which initiates in the nucleus pulposus (NP); a hydrated tissue primarily composed of type 2 collagen and proteoglycan. Current therapies for IVD degeneration (IDD) are palliative and merely delay surgical management. While these procedures may relieve pain temporarily, they fail to address the underlying cause of degeneration and make no attempt to regenerate the effected tissue. Additional concerns with the use …
Analysis Of Citrate Content In Bone Using High Performance Liquid Chromatography, Matthew Konstantin Pysh
Analysis Of Citrate Content In Bone Using High Performance Liquid Chromatography, Matthew Konstantin Pysh
All Theses
Approximately 1.5 wt. % of bone is comprised of citrate molecules bound to the surface of apatite crystals. Furthermore, 80 to 90% of physiological citrate is contained within bone. Recently, interest in citrate has increased due to the potential of a citrate based method for estimation of postmortem intervals, time since death, of skeletal remains. The broad objective of this research was to develop and validate a High-Performance Liquid Chromatography (HPLC) method for quantitative and qualitative analysis of citrate in bone. An appropriate HPLC column and operating conditions for the detection of citrate were selected and subsequently used to analyze …
Effects Of Growth Factor Supplementation And Environmental Conditions On Human Adipose-Derived Stem Cell Differentiation Towards Urothelial Lineage, James Turner
All Dissertations
In recent years, tissue engineering of the bladder has undergone many technological advances. Autologous urothelial cells have been used in animal models and have shown increased performance for ureteral reconstruction compared to unseeded grafts. However, since patients may lack a reliable source of native urothelial cells due to the nature of their specific bladder disease, autologous cells are not an ideal source clinically, and an alternate cell source must be explored. Adipose derived stem cells (ADSCs) are an attractive cell source for such regenerative medicine applications as they have been extensively studied for their multipotential differentiation, immunosuppressive properties, ease of …
Manipulation Of The Electrical Double Layer For Control And Sensing In A Solid State Nanopore, Samuel L. Bearden
Manipulation Of The Electrical Double Layer For Control And Sensing In A Solid State Nanopore, Samuel L. Bearden
All Dissertations
Nanopores have been explored with the goal of achieving non-functionalized, sub-molecular sensors, primarily with the purpose of producing fast, low-cost DNA sequencers. Because of the nanoscale volume within the nanopore structure, it is possible to isolate individual molecular and sub-molecular analytes. Nanopore DNA sequencing has remained elusive due to high noise levels and the challenge of obtaining single-nucleotide resolution. However, the complete electrical double layer within the nanopore is a key feature of fluid-nanopore interaction and has been neglected in previous studies. By exploring interactions with the electrical double layer in various nanopore systems, we characterize the material, electrical, and …
Understanding The Fusion And Maturation Of Tissue Engineered Linear Blood Vessels Using Magnetic Cellular Spheroids, Timothy R. Olsen
Understanding The Fusion And Maturation Of Tissue Engineered Linear Blood Vessels Using Magnetic Cellular Spheroids, Timothy R. Olsen
All Dissertations
Cellular spheroids are attractive for tissue fabrication due to having precise control over cell and extracellular matrix (ECM) composition, the ability for upscaled production and repeatability, their three-dimensional nature and the fact that spheroids will produce their own ECM over time. A critical process in the fabrication of complex tissue structures with cellular spheroids is related to their fusion and maturation. Tissue fusion is a self-assembly process in which two or more distinct cell populations, or tissues, make contact and coalesce to form a single cohesive structure. Maturation of tissue engineered constructs involves developing the mechanical properties and ECM compositions …
Assessment Of Bore-Cone Taper Junctions In Explanted Modular Total Hip Replacements, Christine Margaret Stamer
Assessment Of Bore-Cone Taper Junctions In Explanted Modular Total Hip Replacements, Christine Margaret Stamer
All Theses
For people with chronic hip pain due to arthritis or other conditions, a total hip replacement (THR) is a common procedure used to eliminate the pain. Due to the natural variation in patient anatomy, THR prostheses are becoming increasingly more modular to allow for a more customized initial replacement and for an easier revision if needed in the future. Currently, THR prostheses routinely have modular femoral heads to provide surgeons with intraoperative flexibility. This modularity allows the surgeons to maintain the proper leg length and restore hip biomechanics for the patient and insert the components more easily and precisely. Modular …
Endothelialization Using Protein Substrates And Hydrogels For Aortic Valve Applications, Elizabeth A. Fontaine
Endothelialization Using Protein Substrates And Hydrogels For Aortic Valve Applications, Elizabeth A. Fontaine
All Theses
Aortic valve disease is currently the leading cause of cardiovascular morbidity in the United States.1 In 2003, approximately 290,000 patients required heart valve replacement and that number is estimated to continue to rise as the average age of the population continues to increase.2 However, the current valve replacement options have multiple limitations. Tissue engineering hopes to address these shortcomings by providing a viable valve that more closely mimics the native valve, structurally and functionally.3 An ideal valve replacement should contain endothelial and interstitial cells, with the ability to remodel the extra-cellular matrix. While tissue-engineered heart valves are not uncommon in …