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Biomedical Engineering and Bioengineering Commons™
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- Tissue engineering (3)
- Membrane (2)
- Muscle regeneration (2)
- Scaffold (2)
- Volumetric muscle loss (2)
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- Artificial Photosynthesis (1)
- Bio-solarcell (1)
- Biomaterial (1)
- Biomaterials (1)
- Biopharmaceutical (1)
- Bioreactor clarification (1)
- Bioseparation (1)
- Cell environment (1)
- Cell survival (1)
- Cell-based therapy (1)
- Cellulose Nanocrystals (1)
- Cellulose Nanofibers (1)
- Chemical vapor deposition (1)
- Collagen Binding Domains (1)
- Decellularization (1)
- Device (1)
- Downstream Process (1)
- E. coli hosts (1)
- Extracellular Matrix (1)
- Extracellular matrix (1)
- Fed-Batch (1)
- Fibroblast Growth Factor (1)
- Fouling (1)
- Fusion Protein (1)
- Graphene (1)
Articles 1 - 12 of 12
Full-Text Articles in Biomedical Engineering and Bioengineering
Polymeric Biomaterials Approaches For Engineering The In Vitro Cellular Microenvironment For Mscs, Mahsa Letter-Mahsa Haseli
Polymeric Biomaterials Approaches For Engineering The In Vitro Cellular Microenvironment For Mscs, Mahsa Letter-Mahsa Haseli
Graduate Theses and Dissertations
Cell therapy is a technology that relies on replacing diseased or dysfunctional cells with healthy functioning ones. One of the cells used for such advanced therapies are stem cells, owing to their ability to differentiate into specific cells required for repairing damaged or defective tissues or cells. The majority of cell-based products are intended to transiently persist in the patient, secreting factors which then allow the patient’s body to heal; in these products, the cells are subsequently eliminated from the body. Furthermore, unique manufacturing platforms, in addition to novel commercialization strategies, will be required to create a successful, sustainable cell …
Fabrication And Non-Covalent Functionalization And Characterization Of Graphene-Based Devices On Novel Substrate Cadmium Trithiophosphate (Iv) — Cdps, Abayomi Omotola Omolewu
Fabrication And Non-Covalent Functionalization And Characterization Of Graphene-Based Devices On Novel Substrate Cadmium Trithiophosphate (Iv) — Cdps, Abayomi Omotola Omolewu
Graduate Theses and Dissertations
With graphene at the center of several application areas such as sensing, circuits, high-frequency devices for communication systems, etc., it is crucial to understand how the intrinsic properties of devices made from graphene and other materials like platinum and palladium nanoparticles affect the performance of such devices for the specific application area. Many graphene-based devices for different application areas have focused mainly on the material composition of the graphene-based devices and how it affects performance parameters for the specific application. However, it would be insightful to understand how the intrinsic electrical properties of the graphene devices for different applications affect …
Performance And Fouling During Bioreactor Harvesting, Da Zhang
Performance And Fouling During Bioreactor Harvesting, Da Zhang
Graduate Theses and Dissertations
Tangential flow filtration has many advantages for bioreactor harvesting as the permeate could be introduced directly to the subsequent capture step, the process is easy to scale up, and fouling of the filter is limited by the cross flow. However, membrane fouling has limited its widespread use. This is particularly problematic given the high cell densities encountered today. Here a reverse asymmetric commercial membrane, BioOptimal™ MF-SL (Asahi Kasei), where the more open surface faces the feed stream, and the tighter barrier layer faces the permeate stream, has been investigated for bioreactor harvesting. The open surface contains pores up to 40 …
Synthesis And Performance Testing Of Ecm Fiber Scaffolds, Cassandra Reed
Synthesis And Performance Testing Of Ecm Fiber Scaffolds, Cassandra Reed
Graduate Theses and Dissertations
The progression of regenerative medicine has advanced the treatment of multiple illnesses and injuries throughout the years. A good example of the benefits of this research is the work that has gone into volumetric muscle loss (VML), where more than 20% of the muscle is loss. Skeletal muscle makes up 40% of the human body so a loss of that size greatly diminishes the strength, the flexibility, physiology, and quality of life of the injured individual. For that reason, various techniques are used to counteract the loss of structure and innate cellular signaling in order to circumvent that from happening. …
Evaluating The Effects Of Wood Source On The Physicochemical Properties Of Crosslinked Cellulose Nanocrystals, Helena Tchoungang Nkeumen
Evaluating The Effects Of Wood Source On The Physicochemical Properties Of Crosslinked Cellulose Nanocrystals, Helena Tchoungang Nkeumen
Graduate Theses and Dissertations
Cellulose is an abundant and naturally occurring biopolymer that has been used by humans for food, shelter, and clothing for about two centuries now. Highly crystalline nanoparticles derived from cellulose, called cellulose nanocrystals (CNCs), show great potential to meet the rising need for sustainable and nontoxic materials for biomedical applications. However, multiple biomedical applications of CNCs, such as those involving their use in tissue engineering scaffolds, require CNC-based structures to be stable in aqueous environments, a property that native CNCs do not possess due to their inherent hydrophilicity. Chemical crosslinking of CNCs addresses this issue by providing aqueous stability to …
Peptoid-Based Microsphere Coatings For Biomaterial Applications, Jesse Leland Roberts
Peptoid-Based Microsphere Coatings For Biomaterial Applications, Jesse Leland Roberts
Graduate Theses and Dissertations
Peptoids are peptidomimetic oligomers that predominantly harness similarities to peptides for biomimetic functionality. The incorporation of chiral, aromatic side chains in the peptoid sequence allows for the formation of distinct secondary structures and self-assembly into supramolecular assemblies, including microspheres. Peptoid microspheres can be coated onto substrates for potential use in biosensor technologies, tissue engineering platforms, and drug-delivery systems. They have the potential for use in biomedical applications due to their resistance to proteolytic degradation and low immunogenicity. This dissertation focuses on the physical characteristics and robustness of the peptoid microsphere coatings in various physiological conditions, along with their ability to …
The Effects Of Solution Condition On Virus Filtration Performance, Fnu Namila
The Effects Of Solution Condition On Virus Filtration Performance, Fnu Namila
Graduate Theses and Dissertations
Virus filtration is an integral part of the downstream purification of mammalian cell culture-derived biotherapeutics to assure the viral safety of the products. Virus filtration membranes remove viruses based on a size-exclusion mechanism. Commercial parvovirus filers possess unique membrane structure and are designed to remove smaller non-enveloped parvoviruses with size 18-26 nm. However, some filters face issues, such as pre-mature fouling, the decline of filtrate flux, and reduction in virus retention. This doctoral dissertation focused on identifying the factors that influence the filtrate flux and the virus retention capability of commercial virus filters. The effects of solution pH and ionic …
Production And Purification Of Basic Fibroblast Growth Factor Fused To Two Collagen Binding Domains Expressed In E. Coli Bl21 Using Flask And Fed-Batch, Hazim Aljewari
Graduate Theses and Dissertations
Delivering effective and non-toxic doses of bioactive materials that can aid in activating tissue regeneration to wounded tissue has proven to be an enormous challenge. This study was designed to produce a potential therapeutic recombinant protein by fusing two collagen binding domains to basic fibroblast growth factors (bFGF) through a collagenase cleavage site linker, so it can release the bFGF in a wound site by the action of this enzyme. The novel fusion protein was expressed in Escherichia coli BL-21 (E. coli) using traditional flask shaker and fed-batch cultivation. Cell lysate was purified by FPLC using Immobilized metal affinity chromatography …
Optimized Production And Evaluation Of Cellulose Nanocrystals Derived From Pre-Extracted Kraft Pulp Of Different Wood Species, Gurshagan Kandhola
Optimized Production And Evaluation Of Cellulose Nanocrystals Derived From Pre-Extracted Kraft Pulp Of Different Wood Species, Gurshagan Kandhola
Graduate Theses and Dissertations
Production of nanocellulose from a variety of naturally abundant, locally available and industrially significant wood species provides an opportunity for diversifying the portfolio of traditional pulp and paper industries. The U.S. has a prolific forest products industry with a well-established infrastructure that could be utilized for optimized and customized production of cellulose nanomaterials. However, to achieve that, it is important to a) understand how biorefining strategies for complete fractionation of biomass affect the downstream processing of pulp into nanocellulose, b) maximize the yields of cellulose nanocrystals and nanofibers (CNCs and CNFs) from pretreated raw materials, and c) evaluate if the …
Bioengineering Extracellular Matrix Scaffolds For Volumetric Muscle Loss, Kevin Roberts
Bioengineering Extracellular Matrix Scaffolds For Volumetric Muscle Loss, Kevin Roberts
Graduate Theses and Dissertations
Volumetric muscle loss overwhelms skeletal muscle’s ordinarily capable regenerative machinery, resulting in fibrosis and severe functional deficits which have defied clinical repair strategies. My work spans the design and preclinical evaluation of implants intended to drive the cell community of injured muscle toward a regenerative state, as well as the development of an understanding of the molecular responses of this cell community to biomaterial interventions. I demonstrate a new class of biomaterial by leveraging the productive capacity of sacrificial hollow fiber membrane cell culture; I show specifically that unique threads of whole extracellular matrix can be isolated by solvent degradation …
Characterization Of Bacteriorhodopsin And Halorhodopsin Reconstituted In Lipid Bilayer Membranes, Joel Domkam Kamwa
Characterization Of Bacteriorhodopsin And Halorhodopsin Reconstituted In Lipid Bilayer Membranes, Joel Domkam Kamwa
Graduate Theses and Dissertations
Motivated to produce electricity with photon activated ion pumps, the main purpose of this work was to characterize the photosynthetic membrane proteins bacteriorhodopsin (proton pump) and halorhodopsin (chloride pump). The proteins were re-suspended in lipid bilayers. For this work, an experimental set-up was built which included: chambers for lipid bilayer formation and characterization, lasers for ion pump activation, and an AxoPatch electrophysiology system for small photocurrent measurement. Lipid bilayer membranes were formed using mostly folding method: folding two monolayers together. The membranes were characterized by their resistance, capacitance, and generated photocurrent. Photocurrent was generated upon illumination of lipid-protein membranes with …
Development Of A Novel Device For The Perfusion Driven Decellularization Of Skeletal Muscle, Benjamin Kasukonis
Development Of A Novel Device For The Perfusion Driven Decellularization Of Skeletal Muscle, Benjamin Kasukonis
Graduate Theses and Dissertations
Decellularization of skeletal muscle is a process that removes cellular components of skeletal muscle tissue while leaving behind the intact extracellular matrix (ECM). Skeletal muscle ECM is currently being studied as a biologic scaffold for repairing volumetric muscle loss (VML) because the removal of cells greatly reduces the antigenicity of the donor tissue. Decellularization usually relies on passive diffusion of detergents, surfactants and/or osmotic solutions to strip cells from the ECM. However, passive diffusion alone is usually not sufficient for complete removal of cells from the interior of large pieces of skeletal muscle using detergents, such as sodium dodecyl sulfate …