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Biomedical Engineering and Bioengineering Commons™
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Full-Text Articles in Biomedical Engineering and Bioengineering
The Effect Of Hyperthermia On Doxorubicin Therapy And Nanoparticle Penetration In Multicellular Ovarian Cancer Spheroids, Abhignyan Nagesetti
The Effect Of Hyperthermia On Doxorubicin Therapy And Nanoparticle Penetration In Multicellular Ovarian Cancer Spheroids, Abhignyan Nagesetti
FIU Electronic Theses and Dissertations
The efficient treatment of cancer with chemotherapy is challenged by the limited penetration of drugs into the tumor. Nanoparticles (10 – 100 nanometers) have emerged as a logical choice to specifically deliver chemotherapeutics to tumors, however, their transport into the tumor is also impeded owing to their bigger size compared to free drug moieties. Currently, monolayer cell cultures, as models for drug testing, cannot recapitulate the structural and functional complexity of in-vivo tumors. Furthermore, strategies to improve drug distribution in tumor tissues are also required. In this study, we hypothesized that hyperthermia (43°C) will improve the distribution of silica nanoparticles …
Antibody-Nanoparticle Conjugates To Enhance The Sensitivity Of Elisa-Based Detection Methods., Margaret M Billingsley, Rachel S. Riley, Emily S Day
Antibody-Nanoparticle Conjugates To Enhance The Sensitivity Of Elisa-Based Detection Methods., Margaret M Billingsley, Rachel S. Riley, Emily S Day
Henry M. Rowan College of Engineering Faculty Scholarship
Accurate antigen detection is imperative for clinicians to diagnose disease, assess treatment success, and predict patient prognosis. The most common technique used for the detection of disease-associated biomarkers is the enzyme linked immunosorbent assay (ELISA). In an ELISA, primary antibodies are incubated with biological samples containing the biomarker of interest. Then, detectible secondary antibodies conjugated with horseradish peroxidase (HRP) bind the primary antibodies. Upon addition of a color-changing substrate, the samples provide a colorimetric signal that directly correlates to the targeted biomarker concentration. While ELISAs are effective for analyzing samples with high biomarker content, they lack the sensitivity required to …