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Biomedical Engineering and Bioengineering Commons™
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Full-Text Articles in Biomedical Engineering and Bioengineering
Ultrastructural, Cytochemical, And Immunocytochemical Studies On Bone And Its Interfaces, M. D. Mckee, A. Nanci
Ultrastructural, Cytochemical, And Immunocytochemical Studies On Bone And Its Interfaces, M. D. Mckee, A. Nanci
Cells and Materials
Bone cells possess the ability to synthesize, secrete and direct the assembly and maintenance of extracellular matrix to form a functionally rigid and/or weight-bearing mineralized tissue complex, the skeleton. The skeleton not only supports and protects the organs and tissues of the body, but also acts as a mineral ion reservoir for maintaining systemic calcium balance (calcium homeostasis). The remarkable biological precision necessary for the formation, turnover and constant adaptation of bone to external mechanical forces is a dynamic process requiring a coordinated cellular effort relying on a variety of cell-cell and cell-matrix/mineral interactions. Indeed, cell-matrix interfaces found in a …
Organic Extracellular Matrix Components At The Bone Cell/Substratum Interface, X. Shen, E. Roberts, S. A. F. Peel, J. E. Davies
Organic Extracellular Matrix Components At The Bone Cell/Substratum Interface, X. Shen, E. Roberts, S. A. F. Peel, J. E. Davies
Cells and Materials
We have recently shown that differentiating bone cells are capable of elaborating, in vitro, a calcified matrix at the interface with several non-biological substrata which is similar to cement lines found in remodelling bone tissue. From previous morphological observations, this matrix appeared free of assembled collagen fibres which indicated that osteogenic cells produce an initial mineralized matrix before overt collagen fibre production. In the work reported herein, we used six antibodies to extracellular matrix components and two conjugates of secondary antibody, fluorescein and colloidal gold, to initiate a preliminary characterization of the organic components at the bone cell substratum …
Immunocytochemical And Lectin-Gold Characterization Of The Interface Between Alveolar Bone And Implanted Hydroxyapatite In The Rat, H. Kawaguchi, M. D. Mckee, H. Okamoto, A. Nanci
Immunocytochemical And Lectin-Gold Characterization Of The Interface Between Alveolar Bone And Implanted Hydroxyapatite In The Rat, H. Kawaguchi, M. D. Mckee, H. Okamoto, A. Nanci
Cells and Materials
The tissue response to implanted hydroxyapatite (HAP) in rat alveolar bone was investigated using morphological and cytochemical approaches. High-resolution immunogold labeling was used to examine the distribution of two non-collagenous bone proteins, osteopontin and osteocalcin, and two plasma proteins, a2HSglycoprotein (fetuin) and albumin. Glycoconjugates were characterized by lectin-gold cytochemistry using Ricinus communis I agglutinin and Wheat germ agglutinin. Transmission electron microscopy demonstrated the presence of an electron-dense, lamina limitans-like layer at the HAP particle-bone interface and organic material within the micropores of the particles. Mineral was observed in relation to both these sites. The HAP particle-bone interface was strongly …