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Biomedical Engineering and Bioengineering Commons™
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Full-Text Articles in Biomedical Engineering and Bioengineering
Mineralized Matrix Synthesis By Isolated Mouse Odontoblast-Like Cells In Vitro, P. B. Andrews, A. R. Ten Cate, J. E. Davies
Mineralized Matrix Synthesis By Isolated Mouse Odontoblast-Like Cells In Vitro, P. B. Andrews, A. R. Ten Cate, J. E. Davies
Cells and Materials
First mandibular molar tooth germs were dissected from 17 day mouse embryos. The dental papilla was isolated using both mechanical separation and enzymatic digestion. The cells of the papilla were then enzymatically disaggregated and cultured in 35 mm polystyrene dishes containing alpha minimum essential medium supplemented with 15 % fetal calf serum, 50 μglml ascorbic acid, 1 o-8 M dexamethasone and 10 mM Na-13-glycerophosphate. The cultures were maintained for 23 days. The cultured cells initially appeared as large flat cells having numerous cell processes. Multilayered cell nodules, distributed randomly in the cultures, were apparent after 5 days. Matrix was visible …
The Effect Of Substratum Roughness On Osteoclast-Like Cells In Vitro, K. Gomi, J. D. De Bruijn, M. Ogura, J. E. Davies
The Effect Of Substratum Roughness On Osteoclast-Like Cells In Vitro, K. Gomi, J. D. De Bruijn, M. Ogura, J. E. Davies
Cells and Materials
Calcium phosphate powders were used to produce three groups of experimental substrata for the culture of primary rat bone marrow cells in conditions which permitted the survival and function of osteoclasts. Each of the three experimental groups were subdivided by differences in substratum surface roughness and following a culture period of 7 to 11 days, the culture units were stained for tartrate-resistant acid phosphatase activity. In all samples both small, sometimes mononuclear, and large multinucleate cells stained positive for tartrate-resistant acid phosphatase activity and the numbers and types of cells were quantified and statistically analyzed. Following histochemical staining the samples …