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Biomedical Engineering and Bioengineering Commons™
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Full-Text Articles in Biomedical Engineering and Bioengineering
Simulation Of Free Radical Reactions In Biology And Medicine: A New Two-Compartment Kinetic Model Of Intracellular Lipid Peroxidation, Charles F. Babbs, Melissa Gale Steiner
Simulation Of Free Radical Reactions In Biology And Medicine: A New Two-Compartment Kinetic Model Of Intracellular Lipid Peroxidation, Charles F. Babbs, Melissa Gale Steiner
Weldon School of Biomedical Engineering Faculty Publications
To explore mechanisms of free radical reactions leading to intracellular lipid peroxidation in living systems, we developed a computational model of up to 109 simultaneous enzymatic and free radical reactions thought to be involved in the initiation, propagation, and termination of membrane lipid peroxidation. Rate constants for the various reactions were obtained from the published literature. The simulation model included a lipid membrane compartment and an aqueous cytosolic compartment, between which various chemical species were partitioned. Lipid peroxidation was initiated by the iron-catalyzed, superoxide-driven Fenton reaction. A "C"-language computer program implemented numerical solution of the steady-state rate equations for concentrations …
Quantitative Effects Of Iron Chelators On Hydroxyl Radical Production By The Superoxide-Driven Fenton Raction, Jean Blair Smith, Joanne C. Cusumano, Charles F. Babbs
Quantitative Effects Of Iron Chelators On Hydroxyl Radical Production By The Superoxide-Driven Fenton Raction, Jean Blair Smith, Joanne C. Cusumano, Charles F. Babbs
Weldon School of Biomedical Engineering Faculty Publications
Iron bound to certain chelators is known to promote the conversion of superoxide radicals (O2) to hydroxyl radicals (HO') by the superoxide-driven Fenton reaction. The production of HO' by various iron chelates was studied using the reaction of dimethyl sulfoxide and HO' to produce methane sulphinic acid. Methane sulphinic acid was quantified by use of a simple colorimetric assay and used to determine the amounts of HO' produced. Superoxide was generated from 200 M hypoxanthine and 0.05 U/ml xanthine oxidase in the presence of 0-100 M iron and 100 M of each chelator. The results of this preliminary investigation illustrate …
Protection From Reperfusion Injury In The Isolated Rat Heart By Postischaemic Deferoxamine And Osypurinol Administration, Stephen F. Badylak, Abby Simmons, John Turek, Charles F. Babbs
Protection From Reperfusion Injury In The Isolated Rat Heart By Postischaemic Deferoxamine And Osypurinol Administration, Stephen F. Badylak, Abby Simmons, John Turek, Charles F. Babbs
Weldon School of Biomedical Engineering Faculty Publications
The Langendorff isolated rat heart preparation was used to determine the effect of oxypurinol, a xanthine oxidase inhibitor, and deferoxamine, an iron binding agent, on the extent of myocardial reperfusion injury after 60 minutes of ischaemia. Thirty rats were divided into three groups of 10. and an isolated heart preparation made from each rat. The isolated hearts were perfused for 15 minutes with a modified Krebs-Henseleit perfusate solution to permit stabilisation of the preparation. Each heart was then subjected to 60 minutes of total ischaemia at 37°C followed by 60 minutes of reperfusion with either saline treated perfusate, oxypurinol treated …