Biomedical Engineering and Bioengineering Commons^™

A Microfluidic Culture For Two Populations Of Dorsal Root Ganglia For Differential Staining, Ishnoor Sidhu

Theses

The goal of this study was to design and fabricate a microfluidic system that can be used to visibly distinguish the two populations of dorsal root ganglia (DRGs) by differential staining. Polydimethylsiloxane (PDMS) is the most widely used silicon-based organic polymer, and is particularly known for its wide spread use in microfluidics. Various methods have been employed to pump fluids in these channels for applications ranging from patterning of cells and biomolecules to control of local environment factors such as temperature, which requires external pumping or other applied forces. We demonstrated a pump-free device that exploits the surface energy stored …