Biomedical Engineering and Bioengineering Commons^™

Detection And Neutralization Of Bacterial Biohazards Via Nanoencapsulation, Rosaura De Zayas

FIU Electronic Theses and Dissertations

Bacteria biohazards, such as Anthrax, are responsible for causing mild to serious illnesses in humans and animals. The primary aim of this research study was to develop a rapid one step assay to detect and neutralize bacteria-based biohazards, using an Immunoliposome-nanoparticle complex. An Anthrax model, Bacillus cereus, was grown for 3 hours and diluted 1:25 in media (2.0 x 107 cfu/ml). The Bacillus cereus was interacted with an Immunoliposome-nanoparticle complex containing an MgO-C 2 neutralization agent. The samples were analyzed via flow cytometry with a 1:8:1 ratio Bacillus cereus, Immunoliposome-nanoparticle complex, and Ethidium homodimer-1 for two hours. The results obtained …

Vascular Reactivity Of Isolated Rat Mesenteric Arterioles In The Presence And Absence Of Ouabain, Rohit Chawla

FIU Electronic Theses and Dissertations

The microvasculature plays a significant role in the regulation of blood pressure and regional blood supply. Cardiotonic steroids like the adrenal cortical hormone (ouabain) have been proposed to play a role in some forms of hypertension. The purpose of this study was to determine the effect of different agonists on arteriolar diameter in the presence and absence of ouabain.

In Vitro studies on isolated intact rat mesenteric arterioles were performed by administering different concentrations of the vasoconstrictor norepinephrine (NE) and the vasorelaxant acetylcholine (Ach) in the presence and absence of ouabain. NE induced constriction was not significantly enhanced in the …