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Full-Text Articles in Biomedical Engineering and Bioengineering
Detecting A Heterogenous Sample Of Pigmented Melanoma Cell Lines Using Photoacoustic Flow Cytometry, Margaret Cappellano
Detecting A Heterogenous Sample Of Pigmented Melanoma Cell Lines Using Photoacoustic Flow Cytometry, Margaret Cappellano
Electronic Theses and Dissertations
Metastatic melanoma is the deadliest form of skin cancer, which is in part, attributed to its rapid aggression and lack of response to typical treatment methods. There are far too often cases where a lymph node biopsy does not detect the severity of the cancer, which in turn causes a lack of diagnosis until a mass can be visually detected on a scan, such as a PET, CT, or MRI. Once visible on a scan, the cancer is too progressive for successful treatment. To avoid this, we investigated how a blood sample can be used to negate a missed diagnosis, …
Design, Construction And Application Of A Home-Built, Two-Photon Microscope, William P. Breeding
Design, Construction And Application Of A Home-Built, Two-Photon Microscope, William P. Breeding
Electronic Theses and Dissertations
Two-photon microscopy (TPM) is a powerful, versatile imaging modality for the study of biological systems. This thesis overviews the relevant physics involved in TPM, design considerations and process of constructing a home-built, two-photon microscope, and provides a set of procedures to operate the system. Furthermore, this work explores several applications of TPM through the study of single-cell metabolism and imaging the cellular-material interface. Explored in particular depth was the imaging of cellulose nanofiber (CNF) materials, with the goal of understanding the three-dimensional nature of fibroblast cell growth when embedded within the materials. This work uncovered several optical properties of CNF, …
Development Of Spectroscopic Methods For Dynamic Cellular Level Study Of Biochemical Kinetics And Disease Progression, Anna M. Sitarski
Development Of Spectroscopic Methods For Dynamic Cellular Level Study Of Biochemical Kinetics And Disease Progression, Anna M. Sitarski
Electronic Theses and Dissertations
One of the current fundamental objectives in biomedical research is understanding molecular and cellular mechanisms of disease progression. Recent work in genetics support the stochastic nature of disease progression on the single cell level. For example, recent work has demonstrated that cancer as a disease state is reached after the accumulation of damages that result in genetic errors. Other diseases like Huntingtons, Parkinsons, Alzheimers, cardiovascular disease are developed over time and their cellular mechanisms of disease transition are largely unknown. Modern techniques of disease characterization are perturbative, invasive and fully destructive to biological samples. Many methods need a probe or …