Biomedical Engineering and Bioengineering Commons^™

Role Of Mitochondrial Electron Transport Complex I In Coenzyme Q1 Reduction By Intact Pulmonary Arterial Endothelial Cells And The Effect Of Hyperoxia, Marilyn P. Merker, Said H. Audi, Brian J. Lindemer, Gary S. Krenz, Robert D. Bongard

Biomedical Engineering Faculty Research and Publications

The objective was to determine the impact of intact normoxic and hyperoxia-exposed (95% O(2) for 48 h) bovine pulmonary arterial endothelial cells in culture on the redox status of the coenzyme Q(10) homolog coenzyme Q(1) (CoQ(1)). When CoQ(1) (50 microM) was incubated with the cells for 30 min, its concentration in the medium decreased over time, reaching a lower level for normoxic than hyperoxia-exposed cells. The decreases in CoQ(1) concentration were associated with generation of CoQ(1) hydroquinone (CoQ(1)H(2)), wherein 3.4 times more CoQ(1)H(2) was produced in the normoxic than hyperoxia-exposed cell medium (8.2 +/- 0.3 and 2.4 +/- 0.4 microM, …