Biomedical Engineering and Bioengineering Commons^™

Effect Of Chronic Hyperoxic Exposure On Duroquinone Reduction In Adult Rat Lungs, Said H. Audi, Robert D. Bongard, Gary S. Krenz, David A. Rickaby, Steven Thomas Haworth, Jessica Eisenhauer, David L. Roerig, Marilyn P. Merker

Biomedical Engineering Faculty Research and Publications

NAD(P)H:quinone oxidoreductase 1 (NQO1) plays a dominant role in the reduction of the quinone compound 2,3,5,6-tetramethyl-1,4-benzoquinone (duroquinone, DQ) to durohydroquinone (DQH₂) on passage through the rat lung. Exposure of adult rats to 85% O₂ for ≥7 days stimulates adaptation to the otherwise lethal effects of >95% O₂. The objective of this study was to examine whether exposure of adult rats to hyperoxia affected lung NQO1 activity as measured by the rate of DQ reduction on passage through the lung. We measured DQH₂ appearance in the venous effluent during DQ infusion at different concentrations into …