Biomedical Engineering and Bioengineering Commons^™

Cardioprotection By Glucose-Insulin-Potassium: Dependence On KAtp Channel Opening And Blood Glucose Concentration Before Ischemia, John F. Ladisa, John G. Krolikowski, Paul S. Pagel, David C. Warltier, Judy R. Kersten

Biomedical Engineering Faculty Research and Publications

We tested the hypothesis that glucose-insulin-potassium (GIK)-induced protection against myocardial infarction depends on ATP-dependent K⁺ (K_ATP) channel activation and is abolished by hyperglycemia before the ischemia. Dogs were subjected to a 60-min coronary artery occlusion and 3-h reperfusion in the absence or presence of GIK (25% dextrose; 50 IU insulin/l; 80 mM/l KCl infused at 1.5 ml·kg⁻¹·h⁻¹) beginning 75 min before coronary artery occlusion or 5 min before reperfusion. The role of K_ATP channels was evaluated by pretreatment with glyburide (0.1 mg/kg). The efficacy of GIK was investigated with increases in blood …