Open Access. Powered by Scholars. Published by Universities.®
Biomedical Engineering and Bioengineering Commons™
Open Access. Powered by Scholars. Published by Universities.®
- Discipline
Articles 1 - 2 of 2
Full-Text Articles in Biomedical Engineering and Bioengineering
Live Cell Imaging Heated Stage, Kerri Byrne, Anna Frauenheim, Simon Park
Live Cell Imaging Heated Stage, Kerri Byrne, Anna Frauenheim, Simon Park
Biomedical Engineering
Live cell imaging is a method used by scientists to obtain a better understanding of tissue and cell functions through the study and imaging of cellular dynamics, however it is often detrimental to cell cultures due to effects of changing environment on dynamic cellular events. In order to combat the effects of a changing environment, our team has designed, manufactured, and tested an incubation chamber that will control the heat and pH environment that these cells are experiencing throughout the imaging process. This project is sponsored by the BMED microscopy core group to be used with the Olympus IX73P2F inverted …
Hypoxic Incubation Chamber, Makenzie Jones, Andrea Skattenborg, Henry Elsner
Hypoxic Incubation Chamber, Makenzie Jones, Andrea Skattenborg, Henry Elsner
Biomedical Engineering
In developing a model to test anti-tumor drugs, Dr. Christopher Heylman’s lab requires the ability to culture cells in a hypoxic environment. The purpose of this project was to make this possible. Funding for this project comes from the Biomedical Engineering Department, the Hannah-Forbes Fund, and Dr. Heylman’s lab. The chamber must be able to reach a user-defined oxygen concentration and hold that concentration for 48 hours while creating an environment conducive to cell culture. Importantly, the chamber must also be sterilizable and cleanable for repeated use. While several products exist to create hypoxic environments for cell culture, they are …