Open Access. Powered by Scholars. Published by Universities.®
Biomedical Engineering and Bioengineering Commons™
Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- Bassianolide (1)
- BbBEAS (1)
- BbBSLS (1)
- Beauvericins (1)
- Biosynthesis (1)
-
- Cyclase (1)
- Depsipeptide (1)
- Fungal cyclooligomer (1)
- Fungal cyclooligomer depsipeptide synthetases (1)
- Heterologous expression (1)
- Iterative catalysis (1)
- Ketoisovalerate reductase (1)
- Module swapping (1)
- Natural products (1)
- Phenylalanine ammonia-lyase • reporter assay • curcuminoid biosynthesis • type III polyketide synthase • dicinnamoylmethane (1)
- Polyketides (1)
- Saccharomyces cerevisiae (1)
- Synthetases (1)
Articles 1 - 4 of 4
Full-Text Articles in Biomedical Engineering and Bioengineering
Functional Dissection And Module Swapping Of Fungal Cyclooligomer Depsipeptide Synthetases, Dayu Yu, Fuchao Xu, David Gage, Jixun Zhan
Functional Dissection And Module Swapping Of Fungal Cyclooligomer Depsipeptide Synthetases, Dayu Yu, Fuchao Xu, David Gage, Jixun Zhan
Biological Engineering Faculty Publications
BbBSLS and BbBEAS were dissected and reconstituted in Saccharomyces cerevisiae. The intermodular linker is essential for the reconstitution of the separate modules. Module 1 can be swapped between BbBEAS and BbBSLS, while modules 2 and 3 control the product profiles. BbBSLS is a flexible enzyme that also synthesizes beauvericins.
Engineered Production Of Fungal Anticancer Cyclooligomer Depsipeptides In Saccharo-Myces Cerevisiae, Dayu Yu, Fuchao Xu, Jiachen Zi, Siyuan Wang, David Gage, Jia Zeng, Jixun Zhan
Engineered Production Of Fungal Anticancer Cyclooligomer Depsipeptides In Saccharo-Myces Cerevisiae, Dayu Yu, Fuchao Xu, Jiachen Zi, Siyuan Wang, David Gage, Jia Zeng, Jixun Zhan
Biological Engineering Faculty Publications
Two fungal cyclooligomer depsipeptide synthetases (CODSs), BbBEAS (352 kDa) and BbBSLS (348 kDa) from Beauveria bassiana ATCC 7159, were reconstituted in Saccharomyces cerevisiae BJ5464-NpgA, leading to the production of the corresponding anticancer natural products, beauvericins and bassianolide, respectively. The titers of beauvericins (33.82±1.41 mg/l) and bassianolide (21.74±0.08 mg/l) in the engineered S. cerevisiae BJ5464-NpgA strains were comparable to those in the native producer B. bassiana. Feeding D-hydroxyisovaleric acid (D-Hiv) and the corresponding L-amino acid precursors improved the production of beauvericins and bassianolide. However, the high price of D-Hiv limits its application in large-scale production of these cyclooligomer depsipeptides. Alternatively, we …
Design And Application Of An In Vivo Reporter Assay For Phenylalanine Ammonialyase, S. Wang, S. Zhang, T. Zhou, J. Zeng, Jixun Zhan
Design And Application Of An In Vivo Reporter Assay For Phenylalanine Ammonialyase, S. Wang, S. Zhang, T. Zhou, J. Zeng, Jixun Zhan
Biological Engineering Faculty Publications
Phenylalanine ammonia-lyase (PAL) is an important enzyme that links primary metabolism to secondary metabolism. Its efficiency is often a critical factor that affects the overall flux of a related metabolic pathway, the titer of the final products, and the efficacy of PAL-based therapies. Thus, PAL is a common target for metabolic engineering and it is of significant interest to screen efficient PALs for industrial and medical applications. In this study, a novel and efficient visible reporter assay for screening of PAL efficiency in Escherichia coli was established based on a plant type III polyketide biosynthetic pathway. The candidate PALs were …
Rational Reprogramming Of Fungal Polyketide First Ring Cyclization, Yuquan Xu, Tong Zhou, Zhengfu Zhou, Shiyou Su, Sue A. Roberts, William R. Montfort, Jia Zeng, Ming Chen, Wei Zhang, Min Lin, Jixun Zhan, Istvan Molnar
Rational Reprogramming Of Fungal Polyketide First Ring Cyclization, Yuquan Xu, Tong Zhou, Zhengfu Zhou, Shiyou Su, Sue A. Roberts, William R. Montfort, Jia Zeng, Ming Chen, Wei Zhang, Min Lin, Jixun Zhan, Istvan Molnar
Biological Engineering Faculty Publications
Resorcylic acid lactones (RAL) and dihydroxyphenylacetic acid lactones (DAL) represent important pharmacophores with heat shock response and immune system modulatory activities. The biosynthesis of these fungal polyketides involves a pair of collaborating iterative polyketide synthases (iPKSs): a highly reducing iPKS (hrPKS) whose product is further elaborated by a nonreducing iPKS (nrPKS) to yield a 1,3-benzenediol moiety bridged by a macrolactone. Biosynthesis of unreduced polyketides requires the sequestration and programmed cyclization of highly reactive poly-β-ketoacyl intermediates to channel these uncommitted, pluripotent substrates towards defined subsets of the polyketide structural space. Catalyzed by product template (PT) domains of the fungal nrPKSs and …