Biomedical Engineering and Bioengineering Commons^™

Microfluidic Chip For High Efficiency Microinjection Of Caenorhabditis Elegans, Delaney Gray, Alex Hadsell, Jessica Talamantes

Bioengineering Senior Theses

The terrestrial nematode, Caenorhabditis elegans, is an invaluable model organism for the study of molecular and cellular processes due to their small size, rapid generation time, easy cultivation, and invariant cell number. Additionally, 40% of genes known to be associated with human disease have clear orthologs in the C. elegans genome. In C. elegans genetics research, microinjection of genetic material into the worms is critical. Although an established technique, manual microinjection is tedious, low-throughput, and requires an expert researcher. This thesis details a novel microfluidic device designed to perform high-throughput microinjection. This two-layer, PDMS-based chip integrates microfluidic elements to …

Milkguard: Low-Cost, Polymer-Based Sensor For The Detection Of Escherichia Coli In Donated Human Breast Milk, Maggie May, Nicholas Kikuchi, Matthew Zweber

Bioengineering Senior Theses

Breast milk, the gold standard for infant nutrition, could prevent up to 13% of child deaths worldwide. However, many mothers are unable to breastfeed due to health conditions and other factors. Because of this, a network of more than 500+ human milk banks, which collect and distribute donated breast milk to infants, have emerged worldwide. However, operational costs to ensure the safety of this milk remain time-intensive and costly.

There are no existing diagnostics for rapid and on-site detection of bacterial contaminants in donated milk. Currently, many milk banks send samples to outside laboratories for bacterial culturing tests, which take …