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Biomedical Engineering and Bioengineering Commons™
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Full-Text Articles in Biomedical Engineering and Bioengineering
Automated Methods For Fiber Diameter Measurement Of Fibrous Scaffolds, Anna Bulysheva
Automated Methods For Fiber Diameter Measurement Of Fibrous Scaffolds, Anna Bulysheva
Theses and Dissertations
The purpose of this work was to develop an automated method of measuring fiber diameters of electrospun scaffolds from scanning electron microscopy images of these scaffolds. Several automated methods were developed and evaluated by comparison to known values and data obtained via the standard manual method. Simulated images with known diameters were used as test images to evaluate the accuracy of each measurement technique. Eight scanning electron microscopy images were also used for the evaluation of the automated methods compared to the standard manual method. All diameter measurements were made in pixels. Five new automated methods coded in MATLAB were …
Preparation And Characterization Of An Electrospun Gelatin/Dendrimer Hybrid Nanofiber Dressing, Alicia P. Smith-Freshwater
Preparation And Characterization Of An Electrospun Gelatin/Dendrimer Hybrid Nanofiber Dressing, Alicia P. Smith-Freshwater
Theses and Dissertations
A novel dendritic wound dressing was designed and characterized for its potential to treat chronic wounds. Comprised of gelatin, dendrimer, synthetic polymer and antibiotics, the dressing was electrospun to mimic the natural extracellular matrix (ECM). Gelatin is biocompatible, biodegradable, non-toxic, and easily available. The antibiotic, doxycycline, has the ability to inhibit matrix metalloproteinases. Matrix metalloproteinases, which occur in excess in chronic wounds, degrade the reconstituted ECM. Starburst™ polyamidoamine (PAMAM) dendrimer G3.5, which provides a versatile and structurally controlled architecture to construct nanomedicine, was covalently bonded to the gelatin backbone and electrospun into nanofibers with gelatin, doxycycline and stabilizing polymers. The …
Fabrication And Characterization Of Bioactive, Composite Electrospun Bone Tissue Engineering Scaffolds Intended For Cleft Palate Repair, Parthasarathy Madurantakam
Fabrication And Characterization Of Bioactive, Composite Electrospun Bone Tissue Engineering Scaffolds Intended For Cleft Palate Repair, Parthasarathy Madurantakam
Theses and Dissertations
Tissue Engineering is a scientific discipline that aims to regenerate tissues and organs that are diseased, lost or congenitally absent. It encompasses the use of suitable synthetic equivalents of native extracellular matrix that may or may not be supplemented with cells or relevant growth factors. Such scaffolds are designed to reside at the site of implantation for a variable period of time during which they induce the regeneration of native tissue. During this time, they also provide a template for new cells to attach, infiltrate, differentiate into appropriate phenotype and eventually restore function of the concerned tissue. Among the factors …
Tissue Engineering Cellularized Silk-Based Ligament Analogues, Scott Sell
Tissue Engineering Cellularized Silk-Based Ligament Analogues, Scott Sell
Theses and Dissertations
The resurgence, and eventual rise to prominence in the field of tissue engineering, that electrospinning has experienced over the last decade speaks to the simplicity and adaptability of the process. Electrospinning has been used for the fabrication of tissue engineering scaffolds intended for use in nearly every part of the human body: blood vessel, cartilage, bone, skin, nerve, connective tissue, etc. Diverse as the aforementioned tissues are in both form and function, electrospinning has found a niche in the repair of each due to its capacity to consistently create non-woven structures of fibers ranging from nano-to-micron size in diameter. These …
Structural And Functional Considerations In The Design Of Collagen-Based Electrospun Scaffolds, Chantal Ayres
Structural And Functional Considerations In The Design Of Collagen-Based Electrospun Scaffolds, Chantal Ayres
Theses and Dissertations
Electrospinning can be used to selectively process a variety of natural and synthetic polymers into highly porous scaffolds composed of nano-to-micron diameter fibers. This process shows great potential as a gateway to the development of physiologically relevant tissue engineering scaffolds. In this study we examine the structural and functional considerations regarding electrospun scaffolds for dermal template applications using novel quantification techniques. In order to characterize scaffold structure, a technique utilizing the fast Fourier transform was developed to systematically quantify fiber alignment and evaluate how different electrospinning parameters impact the structure and material properties of an electrospun scaffold. Gelatin was suspended …