Biomedical Engineering and Bioengineering Commons^™

Scanning Electron Microscopy Of The Osteoclast-Bone Interface In Vivo, H. Zhou, R. Chernecky, J. E. Davies

Cells and Materials

Rat femoral bones were studied by scanning electron microscopy to demonstrate the morphology of osteoclast- bone matrix interfacial relationships. Two general morphological types of actively resorbing osteoclasts were observed . One cell type was approximately ovoid with highly fimbriated borders, fully attached and closely adapted to the resorption surface. The ruffled border of such cells was composed of a number of individual filopodia which were of uniform , regular shape and approximately 2-3 μm in length and 150 nm in diameter. They were found to penetrate the bone matrix to a depth of 1 μm and interdigitated with the surrounding …

Projection Effect Errors In Biomaterials And Bone Research, K. N. Bachus, R. D. Bloebaum

Cells and Materials

Micoradiography and backscattered electron (BSE) imaging are techniques used to investigate the morphologic, histometric, and mineral content changes at the bone/biomaterials interface. Investigators have shown that the superimposition of multiple tissue layers can cause errors with both the morphologic observations and the histometric measurements of bone. The objective of this study was to document errors in the bone mineral content measurements associated with overlapping tissues.

Using a digital image analysis system, microradiographic and BSE images from canine cortical and cancellous bone were captured and analyzed. The results of this study showed that microradiography had more projection effect errors associated with …

The Tissue Responses To Tricalcium Phosphate Ceramics In Bone And Marrow, R. S. Archer, S. Downes, M. V. Kayser, S. Y. Ali

Cells and Materials

Calcium phosphate ceramics are described as "bioactive". Evidence of biological activity associated with calcium phosphate implanted in bone and marrow can be seen as changes in the morphology of the interfaces. In this in vivo model the sequence of progressive bone growth and bone merger with a dense calcium phosphate ceramics were examined by light and transmission electron microscopy. A spectrum of tissue responses to dense tricalcium phosphate ceramics was observed at intervals over a six week period. It is now well established that calcium phosphate ceramics permit bone ingrowth with little or no immunological response; however, a layer of …

Composite Filler Characterization By Scanning Electron Microscopy, Image Analysis And Thermogravimetry, J. Vaidyanathan, T. K. Vaidyanathan

Cells and Materials

The morphological and compositional characterization of the filler particles in selected dental composites has been accomplished by a combination of backscattered electron imaging, digital imaging , energy dispersive X-ray analysis and thermogravimetry. Backscattered electron images provide excellent composition contrast between the filler and the matrix in this analysis . Qualitative energy dispersive spectroscopy was used to classify the filler composition in the composites studied. The digital imaging technique of feature analysis was utilized to quantitatively characterize the particle morphological parameters and their distributions. Several parameters were determined of which the particle area, breadth, height and Waddel diameter are emphasized. Particle …