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Full-Text Articles in Biomedical Engineering and Bioengineering
Detecting Physiological Concentrations Of Alzheimer’S Associated Amyloid-Β Protein Utilizing A Cell-Based Response, Brittany Elizabeth Watson
Detecting Physiological Concentrations Of Alzheimer’S Associated Amyloid-Β Protein Utilizing A Cell-Based Response, Brittany Elizabeth Watson
Theses and Dissertations
Alzheimer’s disease (AD) is characterized symptoms of cognitive impairment resulting from blood-brain barrier (BBB) breakdown and neuronal cell death. AD is associated with aggregated amyloid-β (Aβ) plaques deposited around brain vasculature and within the brain. Changes in the brain occur up to 20 years before symptoms arise; however, there is currently no pre-symptomatic detection method available for AD. An intact BBB requires tight junction proteins (TJs) that render relatively high transendothelial electrical resistance (TEER). AD-associated breakdown of the BBB correlates with vascular Aβ deposition, suggesting that aggregated, pathological Aβ can modulate TEER. We explore leveraging this cellular response for early …
Role Of P-Glycoprotein In Alzheimer’S Disease For Enhanced Brain Elimination Of Amyloid-Β, Hope Holt
Role Of P-Glycoprotein In Alzheimer’S Disease For Enhanced Brain Elimination Of Amyloid-Β, Hope Holt
Theses and Dissertations
Alzheimer’s disease (AD), the most common form of neurodegenerative disorder, is characterized by deposition of amyloid-β (Aβ) plaques in the brain. Aβ monomer undergoes nucleation to form oligomers, then soluble aggregates, then fibrils which make up the plaques. Aβ oligomer species are believed to be the most neurotoxic aggregate species. Currently under investigation is a mechanism for Aβ removal from the brain, across the blood-brain barrier (BBB). P-glycoprotein (P-gp) is a membrane-bound efflux protein located on the apical, or blood, side of the BBB, which transports a wide variety of substrates. Further complicating this potential clearance mechanism is the reduction …