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University of Nebraska - Lincoln
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
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Articles 1 - 30 of 48
Full-Text Articles in Biomedical Engineering and Bioengineering
Development Of A Decellularized Hydrogel Composite And Its Application In A Novel Model Of Disc-Associated Low Back Pain In Female Sprague Dawley Rats, David Lillyman
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
Chronic low back pain is a global socioeconomic crisis compounded by an absence of reliable, curative treatments. The predominant pathology associated with chronic low back pain is degeneration of intervertebral discs in the lumbar spine. During degeneration, nerves can sprout into the intervertebral disc tissue and be chronically subjected to inflammatory and mechanical stimuli, resulting in pain. Pain arising from the intervertebral disc, or disc-associated pain, is a complex, multi-faceted disorder which necessitates valid animal models to screen therapeutics and study pathomechanisms of pain.
While many research teams have created animal models of disc degeneration, the translation of these platforms …
Understanding The Role Of Antioxidant Nanoparticles In Improving The Outcome Of Secondary Injury In Traumatic Brain Injury, Aria W. Tarudji
Understanding The Role Of Antioxidant Nanoparticles In Improving The Outcome Of Secondary Injury In Traumatic Brain Injury, Aria W. Tarudji
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
Following traumatic brain injury (TBI), excess reactive oxygen species (ROS) and other free radicals are released, inducing the cascade of secondary injury that exacerbate the outcomes of TBI. Antioxidant nanoparticles (ANPs) have shown promising outcomes in reducing the progression of TBI, which may be due to the higher accumulation and retention of ANPs in the injured brain. However, there is limited knowledge of: 1) antioxidant activities needed in TBI treatment, 2) correlation between longer retention, bioavailability, and target engagement with antioxidant treatments, and 3) sexual dimorphism to ANP treatments.
This dissertation assesses multiple ANPs with various scavenging activities and durations …
Optimization Of A Novel Barnes Maze Protocol For Assessing Antioxidant Treatment Of Traumatic Brain Injury, Connor C. Gee
Optimization Of A Novel Barnes Maze Protocol For Assessing Antioxidant Treatment Of Traumatic Brain Injury, Connor C. Gee
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
Current preclinical research into traumatic brain injury focuses heavily upon cellular and molecular testing to determine the effects of injury and potential benefits of neuroprotective treatments. While this may be a useful method, some argue that an increased focus on behavioral testing could lead to better clinical translation as these assays assess the longer term, downstream effects from a brain injury. The most characterized behavioral tests used in traumatic brain injury research are the spatial learning and memory paradigms, Morris Water Maze and Barnes Maze. The Morris Water Maze is the most used of theses paradigms and relies on spatial …
Development And Characterization Of A Decellularized Neuroinhibitory Scaffold Containing Matrix Bound Nanovesicles, Logan Piening
Development And Characterization Of A Decellularized Neuroinhibitory Scaffold Containing Matrix Bound Nanovesicles, Logan Piening
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
Chronic low back pain (LBP) is a leading cause of disability but treatments for LBP are limited. Degeneration of the intervertebral disc leads to loss of neuroinhibitory sulfated glycosaminoglycans (sGAGs) which allows nerves from dorsal root ganglia (DRG) to grow into the core of the disc, leading to pain. Current treatments for LBP involve drugs that do not target the source of the pain and lack long term efficacy or use invasive surgeries with high complication rates. Treatment with a decellularized tissue scaffold that contains neuroinhibitory components may inhibit nerve growth and prevent disc-associated LBP. Here, a decellularized nucleus pulposus …
Efficient Polyhydroxyalkanoate Production By Rhodopseudomonas Palustris From Lignocellulosic Biomass, Brandi Brown
Efficient Polyhydroxyalkanoate Production By Rhodopseudomonas Palustris From Lignocellulosic Biomass, Brandi Brown
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
Polyhydroxyalkanoates (PHAs) are biopolymers produced by bacteria with the potential to replace conventional plastics. However, the relatively high production costs of PHAs are keeping them from market acceptance, with approximately half of the production costs derived from the feedstock. Thus, engineering a microbe for PHA production from cheaper and renewable carbon sources is necessary to promote the valorization of PHAs. Lignocellulosic biomass is considered to be one of the most economic carbon sources in the world, and is thus an attractive candidate for cheaper production of bioplastics. Rhodopseudomonas palustris CGA009 is a metabolically robust bacterium capable of catabolizing lignin breakdown …
Preclinical Development Of Single Walled Carbon Nanotube-Based Optical Biosensors, Eric M. Hofferber
Preclinical Development Of Single Walled Carbon Nanotube-Based Optical Biosensors, Eric M. Hofferber
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
High resolution, long-term monitoring of key biological analytes would improve patient outcomes by providing earlier detection of disease states and improved efficacy of treatment. One class of biosensors that have gained much attention in recent years are optical biosensors. Optical probes are attractive biosensors due to their noninvasive nature of detection, as certain light can pass through tissue, water, and blood. Single walled carbon nanotubes (SWNT) are a specific type of optical biosensor that fluoresce in the near infrared range of the electromagnetic spectrum and offer unparalleled spatial and temporal resolution. SWNT have been applied as biosensors in vitro, …
Development Of An Internet Of Things (Iot) Enabled Novel Wireless Multi Sensor Network For Infield Crop Monitoring, Nipuna Chamara
Development Of An Internet Of Things (Iot) Enabled Novel Wireless Multi Sensor Network For Infield Crop Monitoring, Nipuna Chamara
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
Multispectral imaging systems on satellite, aerial, and ground platforms are used commonly to monitor in-field crops in precision agriculture by farmers and researchers. Limited spatial and temporal resolution and weather dependence of the data collection are two main disadvantages of these methods. In-field sensor networks can continuously monitor environmental and plant physiological parameters by leveraging low-power computation and long-range communication technologies. We built and tested a novel sensor network equipped with soil moisture, multispectral and RGB imaging sensors in an experimental soybean field at Eastern Nebraska Research and Extension Center, NE, USA. 10 down-looking and 1 up-looking sensor node were …
Enhancing Single Walled Carbon Nanotube Deposition For The Study Of Extracellular Analytes, Joseph A. Stapleton
Enhancing Single Walled Carbon Nanotube Deposition For The Study Of Extracellular Analytes, Joseph A. Stapleton
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
Extracellular signaling is a dynamic process responsible for coordinating large scale biological processes. As such, understanding extracellular signaling is important to our determination of normal function and pathophysiological development. High resolution spatial and temporal information are critical to completely understanding these processes. Unfortunately, current methods of detection are lacking in either spatial or temporal resolution of extracellular products, limiting researchers’ ability to understand complex biological processes. A new group of sensors based on fluorescent single walled carbon nanotubes (SWNT) have shown the potential to provide both high quality spatial and temporal resolution for the sensing of analytes. However, while SWNT …
Design, Development, And Field Testing A Visnir Integrated Multi-Sensing Soil Penetrometer, Nuwan K. Wijewardane
Design, Development, And Field Testing A Visnir Integrated Multi-Sensing Soil Penetrometer, Nuwan K. Wijewardane
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
The research community in soil science and agriculture lacks a cost-effective and rapid technology for in situ, high resolution vertical soil sensing. Visible and near infra-red (VisNIR) technology has the potential to be used for such sensor development due to its ability to derive multiple soil properties rapidly using a single spectrum. Such efforts must, however, overcome a few challenges: (i) a dry ground soil spectral library that can be used to predict the target soil properties accurately, (ii) a robust design which can acquire high quality VisNIR spectra of soil, (iii) an effective method that can link field intact …
Predictive Modeling Of Fate And Transport Of Three Prevalent Contaminants In Midwest Agroecosystem Surface Waters: Nitrate-N, Atrazine, And Escherichia Coli, Samuel Hansen
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
The majority of streams and rivers in the United States (U.S.) are ecologically impaired, or threatened by anthropogenic stressors. Recent reports have found atrazine in drinking water to be associated with increased birth defects and incidences of Non-Hodgkin’s Lymphoma, with higher levels of significance from exposure to both atrazine and nitrate-N. In contrast, recent illnesses from E. coli contaminating vegetables that originated from irrigation water has increased awareness of identifying sources of E. coli entering irrigation reservoirs.
Methods to accurately predict atrazine and E. coli occurrence and potential sources in waterways continue to limit the identifying appropriate and effective prevention …
Chemical And Physical Priming Of Human Mesenchymal Stem Cells To Alter Nonviral Gene Delivery Outcomes, Tyler Kozisek, Andrew Hamann, Amy Mantz, Mathias Schubert, Eva Schubert, Angela K. Pannier
Chemical And Physical Priming Of Human Mesenchymal Stem Cells To Alter Nonviral Gene Delivery Outcomes, Tyler Kozisek, Andrew Hamann, Amy Mantz, Mathias Schubert, Eva Schubert, Angela K. Pannier
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
Human Mesenchymal Stem Cells: Human mesenchymal stem cells (hMSCs) are a multipotent cell, meaning they are able to differentiate into a more mature cell type, such as osteocytes, chondrocytes, and adipocytes, that are found in numerous tissues in the human body, such as bone marrow, fat, and muscle. Since hMSCs can be derived from adult human tissues, they do not have the same ethical concern associated with them as other stem cells, such as embryonic stem cells. Due to hMSCs multipotency and ease of obtaining, they have become one of the most widely researched stem cell types in areas such …
Brain Encoding Of Saltatory Velocity-Scaled Somatosensory Array In Glabrous Hand Among Neurotypical Adults, Hyuntaek Oh
Brain Encoding Of Saltatory Velocity-Scaled Somatosensory Array In Glabrous Hand Among Neurotypical Adults, Hyuntaek Oh
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
Neurons in human somatosensory cortex are somatotopically organized, with sensation from the lower limbs mediated by neurons near the midline of the brain, whereas sensations from the upper body, hands and orofacial surfaces are mediated by neurons located more laterally in a sequential map. Neurons in Brodmann's area (BA) 3b are exquisitely sensitive to tactile stimulation of these skin surfaces. Moreover, the location, velocity and direction of tactile stimuli on the skin's surface are discriminable features of somatosensory processing, however their role in fine motor control and passive detection are poorly understood in health, and as a neurotherapeutic agent in …
Alginate Hydrogels As Three-Dimensional Scaffolds For In Vitro Culture Models Of Growth Plate Cartilage Development And Porcine Embryo Elongation, Taylor D. Laughlin
Alginate Hydrogels As Three-Dimensional Scaffolds For In Vitro Culture Models Of Growth Plate Cartilage Development And Porcine Embryo Elongation, Taylor D. Laughlin
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
The establishment of in vitro culture models utilizes tissue engineering principles to design functional mimics of in vivo environments in vitro. Advantages for the use of in vitro culture models include ethical alleviation of animal models for therapeutic testing, cost efficiency, and a greater ability to study specific mechanisms via a systematic, ground-up approach to development. In this thesis, alginate hydrogels are utilized in the development of in vitro culture models of porcine embryo elongation and growth plate cartilage development. First, the effect of scaffold and modifications to the scaffold were explored in both projects. In order to modulate …
Using A Vnir Spectral Library To Model Soil Carbon And Total Nitrogen Content, Nuwan K. Wijewardane
Using A Vnir Spectral Library To Model Soil Carbon And Total Nitrogen Content, Nuwan K. Wijewardane
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
n-situ soil sensor systems based on visible and near infrared spectroscopy is not yet been effectively used due to inadequate studies to utilize legacy spectral libraries under the field conditions. The performance of such systems is significantly affected by spectral discrepancies created by sample intactness and library differences. In this study, four objectives were devised to obtain directives to address these issues. The first objective was to calibrate and evaluate VNIR models statistically and computationally (i.e. computing resource requirement), using four modeling techniques namely: Partial least squares regression (PLS), Artificial neural networks (ANN), Random forests (RF) and Support vector regression …
High Throughput Screening Of Priming Candidates For Impact On Nonviral Gene Delivery, Albert L. Nguyen
High Throughput Screening Of Priming Candidates For Impact On Nonviral Gene Delivery, Albert L. Nguyen
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
Priming, in the context of nonviral gene delivery, is the treatment of cells with a compound prior to gene transfer that enhances transfection efficiency and/or transgene expression. Essentially, it is the application of an adjuvant approach to gene delivery. Effective transfection strategies may require priming to compete with the efficiency of viral transduction in order to achieve clinically relevant efficiency and expression in vivo. To search for priming compounds, a high throughput screen of the NIH Clinical Collection was performed using 25kDa b-PEI, an EGFP/luciferase plasmid, and HEK293T cells. The EGFP reporter was multiplexed with Hoechst 33342 and Resazurin fluorescence …
Multiphysics Modeling To Enhance Understanding Of Microwave Heating Of Food In Domestic Ovens, Jiajia Chen
Multiphysics Modeling To Enhance Understanding Of Microwave Heating Of Food In Domestic Ovens, Jiajia Chen
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
Nonuniform heating is the biggest issue in the microwave heating of prepared meals. Multiphysics based models are promising tools to improve microwave heating uniformity by properly designing the food product. However, limited availability of accurate temperature-dependent material properties, inadequate model prediction accuracy, and high computational power and complexity in model development are three gaps that greatly limited the application of these models in the food industry.
To fill in the gaps, firstly, we developed a multitemperature calibration protocol to measure temperature-dependent dielectric properties (dielectric constant and loss factor). The temperature-dependent dielectric and thermal (thermal conductivity and specific heat capacity) properties …
Modeling The Contributions Of Hand Loads To Cervical Spine Compressive Forces, Mohamed R.S. Amar
Modeling The Contributions Of Hand Loads To Cervical Spine Compressive Forces, Mohamed R.S. Amar
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
The purpose for this study is threefold. All involve single handed lifting tasks where the height of the lifted object, the distance of the lifted object from the body, and the angle of the lift relative to the body were varied. First, examine the activity of each of the shared musculature of selected shared muscles between the shoulder and cervical spine. Second, attempt to empirically model the activations of the shared muscles. Third, to understand the relationship between the active and antagonistic contraction. Finally, to establish a biomechanical model to estimate the compressive forces on the cervical spine.
Ten subjects …
A Highly Adaptive And Cost Effective Second Generation Incubator (Sgi) Towards Educational, Research And Clinical Processes, Yassir H. Al-Sayagh
A Highly Adaptive And Cost Effective Second Generation Incubator (Sgi) Towards Educational, Research And Clinical Processes, Yassir H. Al-Sayagh
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
Today´s rising demand for more reliable and affordable alternatives to organ transplant has led to a growing market for in vitro tissue culture systems. The main objective of tissue engineering as a whole is to employ human tissue equivalents for commercial use. The state-of-the-art processes for producing these so called tissue models are still very expensive, difficult to produce and time-consuming.
To engineer tissues, in vitro, a three-dimensional support structure commonly termed scaffold is needed. Stem cells are then added to the scaffold. In order for tissue to materialize, the scaffold and the cells must be incubated (cultured) at a …
Applications Of Novel Mri Technologies In Tissue Engineering And Disease Diagnosis, Vahid Khalilzad-Sharghi
Applications Of Novel Mri Technologies In Tissue Engineering And Disease Diagnosis, Vahid Khalilzad-Sharghi
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
Magnetic resonance imaging (MRI) and magnetic resonance elastography (MRE) are increasingly under investigation to explore their potential role in establishing effective evaluation methods for the procedure of tissue regeneration carried out in vitro, in vivo, and in disease diagnosis. To this end, there is a continuous pursuit of novel tools both in vitro and in vivo. For instance, there is a great need for the development and evaluation of an MR-compatible incubation system that enables simultaneous monitoring and culturing of cell and tissue constructs using MRI techniques. Such an imagingcompatible incubation system eliminates exposing the culture to the risks of …
Zein: Novel Natural Polymer For Nanoparticle- And Film-Mediated Gene Delivery, Jessica D. Taylor
Zein: Novel Natural Polymer For Nanoparticle- And Film-Mediated Gene Delivery, Jessica D. Taylor
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
Gene delivery, the introduction of DNA into cells, is applicable to gene therapy, DNA vaccination, functional genomics and diagnostics, tissue engineering, and drug-eluting medical devices. Particulates incorporating DNA are promising vehicles for gene delivery and overcome some of the barriers that hinder successful gene transfer, with the ability to protect DNA and provide for controlled, localized, and sustained release and transfection. Furthermore, innovative new gene delivery strategies that incorporate DNA particulates or complexes within films or coatings for devices and scaffolds could further provide for controlled and sustained transfection at the site of implant. Zein, a hydrophobic protein from corn, …
Morphometric, Biochemical, And Raman Spectral Analyses Of Muscle Damage In Patients With Peripheral Arterial Disease: Providing Objective Criteria For Diagnosis And Treatment Monitoring, Kim Cluff
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
Peripheral arterial disease (PAD), which affects approximately 10 million Americans, is characterized by atherosclerosis of the non-coronary arteries. PAD produces a progressive accumulation of ischemic injury to the limbs that is reflected in a gradual worsening in the myofiber morphology and oxidative damage in the gastrocnemius muscle. In this study, we evaluated the hypothesis that quantitative morphological and biochemical parameters of gastrocnemius myofibers change in a consistent manner during the progression of PAD, provide an objective grading of muscle degeneration in the ischemic limb, and correlate to clinical stage of PAD. Myofiber morphometrics were determined precisely with mathematical equations that …
Two-Dimensional Blood Flow Velocity Estimation Using Ultrasound Speckle Pattern Dependence On Scan Direction And Velocity, Tiantian Xu
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
Cardiovascular disease is the leading cause death in the United States. Although emergency surgery and medicine can be used for cardiovascular disease treatment, the survivors will suffer for it afterwards. Ultrasound blood flow measurement provides a noninvasive way for cardiovascular diseases diagnosis. In this dissertation, three algorithms for blood flow velocity measurements were investigated and optimized. Feature tracking has been previously proposed for vector velocity measurement. In Chapter 3, the optimal amplitude and time thresholds for feature extraction were investigated to minimize flow estimate variance while providing sufficient spatial and temporal coverage of flow area. A new method of lateral …
Dynamics Of Depression Storage During Sprinkler Irrigation And Precipitation Events, Michael B. Mckinney
Dynamics Of Depression Storage During Sprinkler Irrigation And Precipitation Events, Michael B. Mckinney
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
Surface depression storage (Ds) is the volume of precipitation excess which is stored by surface microrelief features of soils. The volume of water stored in surface depressions when precipitation rate exceeds infiltration rate reduces the amount of runoff generated. Because Ds is dependent on soil microrelief, land slope, and crop residue, tillage and management practices can have a considerable impact on the magnitude of this value.
When modeling irrigation systems and surface hydrology, depression storage is often treated as a static abstraction, meaning that maximum storage volume must be filled before runoff occurs. However, several researchers have documented …
Chemical Modification Of Polysaccharides Using Reactive Extrusion, Pratik Bhandari
Chemical Modification Of Polysaccharides Using Reactive Extrusion, Pratik Bhandari
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
The objective of this dissertation was to study the use of reactive extrusion for the chemical modifications of starch and cellulose. A carboxymethyl derivative of starch and carboxymethyl and acetate derivatives of cellulose were prepared using reactive extrusion.
Carboxymethyl starch with rapid swelling properties in water was prepared using reactive extrusion. This was achieved by controlling the gelatinization and through the use of NaOH by controlling the water/ethanol ratio. The effects of NaOH, H2O, temperature, ethanol, sodium mono chloro acetate, sodium tripolyphosphate, citric acid, epichlorohydrin and extruder screw configuration on the degree of substitution of carboxymethyl starch were …
Studies On Power Hop Using A Nonlinear Model Of An Agricultural Tractor, Adam B. Flaugh
Studies On Power Hop Using A Nonlinear Model Of An Agricultural Tractor, Adam B. Flaugh
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
Power hop is an unstable dynamic phenomenon that is observed on agricultural tractors operating at field working speeds under typical draft loads. This dynamic instability can be identified as a bounce motion preceded by an increasing oscillation in either the pitch or bounce modes. A definite and comprehensive correction or prevention strategy for the control of power hop does not exist. In this work a linear and a nonlinear model of an agricultural tractor were developed. The method of multiple time scales has been employed in the analysis of the nonlinear model. Numerical results have been obtained using Mathematica to …
The Economics And Logistics Of The Dual Harvest Of Grain And Biomass In A Single-Pass, Matthew T. Wold
The Economics And Logistics Of The Dual Harvest Of Grain And Biomass In A Single-Pass, Matthew T. Wold
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
Significant interest has developed in using cellulosic resources, especially crop residues, to create biofuels. Collecting these residues in a single-pass of the harvester across the field has the potential to be a low cost option. Two models have been developed; the first characterizes the in-field logistics of single-pass crop residue collection, the second the economics. These models allow the user to easily examine a wide variety of both grain-only and single-pass residue collection harvest cases. A variety of possible residue collection cases have been examined, and their effects both on harvester field capacity and harvest cost compared to grain-only harvest …
Extrusion And Alkali Extrusion Of Corn Stover To Improve Enzyme Saccharification, Shujing Zhang
Extrusion And Alkali Extrusion Of Corn Stover To Improve Enzyme Saccharification, Shujing Zhang
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
Biomass pretreatment constitutes an important part in the whole process of bioethanol production. The main goals of pretreatment include improving cellulose accessibility to enzyme, enhancing sugar yields from hemicellulose, achieving low capital and energy costs, as well as low sugar degradation and low chemical requirements. Extrusion, as a continuous and high throughput pretreatment method, requires much less chemical compared to traditional alkali pretreatment. In this research, twin-screw extrusion was evaluated for its effect with regard to promoting sugar yields from corn stover. Also, the underlying factors contributing to this improvement were examined. It was found that extrusion with no chemicals …
Quantitative Analysis Of The Reaction Between Gliadin And Citric Acid Under Weak Acidic And Weak Alkaline Conditions, Wei Li
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
Gliadin was reacted with citric acid under weak acidic and weak alkaline conditions in both wet and dry states and the reaction mechanism was studied. The low morphological stability in an aqueous environment and inferior mechanical properties have restricted the applications of plant proteins, although these materials possess a unique structure, biocompatibility and biodegradability. Carboxylic acids such as citric acid are inexpensive and nontoxic chemicals and are preferred for crosslinking proteins and cellulose to improve the desired properties of the materials.
In this study, gliadin was chosen as a model of plant proteins to react with citric acid. However, previous …
Zein Nanospheres For Gene Delivery, Mary C. Regier
Zein Nanospheres For Gene Delivery, Mary C. Regier
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
Particulates incorporating DNA provide for protection and sustained release of DNA, and thus are promising candidates for DNA delivery systems. Among the routes of administration for gene delivery, the oral route is perhaps the most appealing as it is associated with patient comfort and compliance and allows for targeting to intestinal targets for therapeutic and vaccination applications. With the goal of realizing the potential of an oral DNA delivery system, zein, a hydrophobic protein from corn that is biocompatible and degraded enzymatically, was investigated. This thesis describes the formulation of zein nanospheres encapsulating DNA by a coacervation technique and their …
Monitoring Of Mesenchymal Based Constructs Using Magnetic Resonance Elastography, Evan T. Curtis
Monitoring Of Mesenchymal Based Constructs Using Magnetic Resonance Elastography, Evan T. Curtis
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
Evaluating the functionality of an engineered material lies in the proper characterization of its material and functional properties. In the treatment of musculoskeletal disorders, engineered bone or fat tissue must behave as an adequate replacement else failure of the material could result in discomfort and further surgical procedures. A significant material characteristic that reflects tissue development is the mechanical properties (i.e. shear strength and viscosity). Shear strength and viscosity provide an indication of how efficient the material is in dissipating energy. Energy dissipation occurs naturally in many tissues including fat and can prevent damage to deeper tissues. Many of the …