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Biomedical Engineering and Bioengineering Commons™
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Articles 1 - 3 of 3
Full-Text Articles in Biomedical Engineering and Bioengineering
Chemical And Physical Priming Of Human Mesenchymal Stem Cells To Alter Nonviral Gene Delivery Outcomes, Tyler Kozisek, Andrew Hamann, Amy Mantz, Mathias Schubert, Eva Schubert, Angela K. Pannier
Chemical And Physical Priming Of Human Mesenchymal Stem Cells To Alter Nonviral Gene Delivery Outcomes, Tyler Kozisek, Andrew Hamann, Amy Mantz, Mathias Schubert, Eva Schubert, Angela K. Pannier
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
Human Mesenchymal Stem Cells: Human mesenchymal stem cells (hMSCs) are a multipotent cell, meaning they are able to differentiate into a more mature cell type, such as osteocytes, chondrocytes, and adipocytes, that are found in numerous tissues in the human body, such as bone marrow, fat, and muscle. Since hMSCs can be derived from adult human tissues, they do not have the same ethical concern associated with them as other stem cells, such as embryonic stem cells. Due to hMSCs multipotency and ease of obtaining, they have become one of the most widely researched stem cell types in areas such …
Alginate Hydrogels As Three-Dimensional Scaffolds For In Vitro Culture Models Of Growth Plate Cartilage Development And Porcine Embryo Elongation, Taylor D. Laughlin
Alginate Hydrogels As Three-Dimensional Scaffolds For In Vitro Culture Models Of Growth Plate Cartilage Development And Porcine Embryo Elongation, Taylor D. Laughlin
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
The establishment of in vitro culture models utilizes tissue engineering principles to design functional mimics of in vivo environments in vitro. Advantages for the use of in vitro culture models include ethical alleviation of animal models for therapeutic testing, cost efficiency, and a greater ability to study specific mechanisms via a systematic, ground-up approach to development. In this thesis, alginate hydrogels are utilized in the development of in vitro culture models of porcine embryo elongation and growth plate cartilage development. First, the effect of scaffold and modifications to the scaffold were explored in both projects. In order to modulate …
Zein: Novel Natural Polymer For Nanoparticle- And Film-Mediated Gene Delivery, Jessica D. Taylor
Zein: Novel Natural Polymer For Nanoparticle- And Film-Mediated Gene Delivery, Jessica D. Taylor
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
Gene delivery, the introduction of DNA into cells, is applicable to gene therapy, DNA vaccination, functional genomics and diagnostics, tissue engineering, and drug-eluting medical devices. Particulates incorporating DNA are promising vehicles for gene delivery and overcome some of the barriers that hinder successful gene transfer, with the ability to protect DNA and provide for controlled, localized, and sustained release and transfection. Furthermore, innovative new gene delivery strategies that incorporate DNA particulates or complexes within films or coatings for devices and scaffolds could further provide for controlled and sustained transfection at the site of implant. Zein, a hydrophobic protein from corn, …