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Chemogenomic Profiling: Identifying The Functional Interactions Of Small Molecules In Yeast, Patrick Flaherty
Chemogenomic Profiling: Identifying The Functional Interactions Of Small Molecules In Yeast, Patrick Flaherty
Patrick Flaherty
We demonstrate the efficacy of a genome-wide protocol in yeast
that allows the identification of those gene products that functionally
interact with small molecules and result in the inhibition of
cellular proliferation. Here we present results from screening 10
diverse compounds in 80 genome-wide experiments against the
complete collection of heterozygous yeast deletion strains. These
compounds include anticancer and antifungal agents, statins, alverine
citrate, and dyclonine. In several cases, we identified previously
known interactions; furthermore, in each case, our analysis
revealed novel cellular interactions, even when the relationship
between a compound and its cellular target had been well established.
In …