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Full-Text Articles in Biomedical Engineering and Bioengineering
Dtaf Dye Concentrations Commonly Used To Measure Microscale Deformations In Biological Tissues Alter Tissue Mechanics., Spencer E Szczesny, Rachel S. Riley, Dawn M Elliott
Dtaf Dye Concentrations Commonly Used To Measure Microscale Deformations In Biological Tissues Alter Tissue Mechanics., Spencer E Szczesny, Rachel S. Riley, Dawn M Elliott
Henry M. Rowan College of Engineering Departmental Research
Identification of the deformation mechanisms and specific components underlying the mechanical function of biological tissues requires mechanical testing at multiple levels within the tissue hierarchical structure. Dichlorotriazinylaminofluorescein (DTAF) is a fluorescent dye that is used to visualize microscale deformations of the extracellular matrix in soft collagenous tissues. However, the DTAF concentrations commonly employed in previous multiscale experiments (≥2000 µg/ml) may alter tissue mechanics. The objective of this study was to determine whether DTAF affects tendon fascicle mechanics and if a concentration threshold exists below which any observed effects are negligible. This information is valuable for guiding the continued use of …