Biomedical Engineering and Bioengineering Commons^™

Protection From Reperfusion Injury In The Isolated Rat Heart By Postischaemic Deferoxamine And Osypurinol Administration, Stephen F. Badylak, Abby Simmons, John Turek, Charles F. Babbs

Weldon School of Biomedical Engineering Faculty Publications

The Langendorff isolated rat heart preparation was used to determine the effect of oxypurinol, a xanthine oxidase inhibitor, and deferoxamine, an iron binding agent, on the extent of myocardial reperfusion injury after 60 minutes of ischaemia. Thirty rats were divided into three groups of 10. and an isolated heart preparation made from each rat. The isolated hearts were perfused for 15 minutes with a modified Krebs-Henseleit perfusate solution to permit stabilisation of the preparation. Each heart was then subjected to 60 minutes of total ischaemia at 37°C followed by 60 minutes of reperfusion with either saline treated perfusate, oxypurinol treated …