Biomedical Engineering and Bioengineering Commons^™

Mg2+ Differentially Regulates Two Modes Of Mitochondrial Ca2+ Uptake In Isolated Cardiac Mitochondria: Implications For Mitochondrial Ca2+ Sequestration, Christoph A. Blomeyer, Jason Bazil, David F. Stowe, Ranjan K. Dash, Amadou Ks Camara

Biomedical Engineering Faculty Research and Publications

The manner in which mitochondria take up and store Ca²⁺ remains highly debated. Recent experimental and computational evidence has suggested the presence of at least two modes of Ca²⁺ uptake and a complex Ca²⁺ sequestration mechanism in mitochondria. But how Mg²⁺ regulates these different modes of Ca²⁺ uptake as well as mitochondrial Ca²⁺ sequestration is not known. In this study, we investigated two different ways by which mitochondria take up and sequester Ca²⁺ by using two different protocols. Isolated guinea pig cardiac mitochondria were exposed to varying concentrations of CaCl₂ in the …