Open Access. Powered by Scholars. Published by Universities.®
Biomedical Engineering and Bioengineering Commons™
Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Biomedical Engineering and Bioengineering
Rna Based Plasmid Selection System For Antibiotic-Free Plasmid Dna Vector Production, Aaron E. Carnes, Jeremy Luke, Justin Vincent, Clague Hodgson, James Williams
Rna Based Plasmid Selection System For Antibiotic-Free Plasmid Dna Vector Production, Aaron E. Carnes, Jeremy Luke, Justin Vincent, Clague Hodgson, James Williams
Vaccine Technology III
Antibiotic resistance markers, typically kanamycin resistance (kanR), allow selective retention of plasmid DNA during bacterial fermentation and are the most commonly utilized selectable markers. However, to ensure safety, regulatory agencies recommend elimination of antibiotic resistance markers from therapeutic and vaccine plasmid DNA vectors. The presence of an antibiotic resistance gene in the plasmid backbone is considered undesirable by regulatory agencies, due to: 1) the potential transfer of antibiotic resistance to endogenous microbial flora; and 2) the potential activation and transcription of the genes from mammalian promoters after cellular incorporation into the genome. Here, we describe the development and application of …
Poly-Methyl Vinyl Ether-Co-Maleic Anhydride Nanoparticles As Antigen Delivery And Activating Systems, Carlos Gamazo, Ibai Tamayo, Ana I. Camacho
Poly-Methyl Vinyl Ether-Co-Maleic Anhydride Nanoparticles As Antigen Delivery And Activating Systems, Carlos Gamazo, Ibai Tamayo, Ana I. Camacho
Vaccine Technology III
The incorporation of antigens into poly-methyl vinyl ether-co-maleic anhydride nanoparticles (NP) has demonstrated to enhance the immune responses in terms of a potent Th1-adjuvant capacity. This fact may be explained by the implemented possibilities that NP render to the antigen: controlled release from the vehicle and chemotaxis for APC recruitment. Besides, after oral administration, it was reported that the bioadhesive nature of the polymer enhanced the interaction of the particulate-adjuvant to the gut mucosa. Moreover, these NP allow the adhesion of antigens and ligands to its outer shell, creating high antigen density surfaces that increase the possibilities of antigen recognition …